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Datasheets/Manuals | Printable datasheet for Goat Anti-ZASP / CYPHER / LDB3 Antibody (OAEB01548) |
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Predicted Species Reactivity | Dog|Human|Mouse|Porcine|Rat |
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Product Format | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Clonality | Polyclonal |
Host | Goat |
Application | WB, IHC |
Additional Information | Reactivity Info: Tested: Human; Expected: Human, Mouse, Rat, Dog, Pig |
Reconstitution and Storage | Aliquot and store at -20C. Minimize freezing and thawing. |
Immunogen | LDB3 |
Purification | Ammonium Sulfate Precipitated; Affinity Purified |
Predicted Homology Based on Immunogen Sequence | Dog: 91%; Horse: 91%; Human: 100%; Mouse: 83%; Pig: 91%; Rabbit: 91%; Rat: 83% |
Peptide Sequence | C-KSQNKPEDEADE |
Concentration | 0.5 mg/ml |
Purity | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Application Info | Peptide ELISA: antibody detection limit dilution 1:2000. Western Blot: Experiments gave bands at approx 80-90kDa and 30-35kDa in Human Skeletal Muscle lysates after 0.01ug/ml antibody staining. These bands correspond to earlier findings in literature with different antibodies (Huang et al J Biol Chem. 2003 Feb 28;278(9):7360-5; PMID: 12499364). This protein has a calculated MW of 77.1kDa according to NP_009009.1. Recommended concentration: 0.01-0.03ug/ml. |
Reference | * Selcen D, Engel AG.; Mutations in ZASP define a novel form of muscular dystrophy in humans.; Ann Neurol. 2005 Feb;57(2):269-76.; Pubmed 15668942 |
Gene Symbol | LDB3 |
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Gene Full Name | LIM domain binding 3 |
Alias Symbols | cardiomyopathy, dilated 1C (autosomal dominant);CMD1C;CMH24;CMPD3;CYPHER;LDB3Z1;LDB3Z4;LIM domain-binding protein 3;LIM domain-binding protein 3 isoform;LVNC3;MFM4;ORACLE;PDLIM6;PDZ and LIM domain 6;protein cypher;ZASP;Z-band alternatively spliced PDZ-motif protein. |
NCBI Gene Id | 11155 |
Protein Name | LIM domain-binding protein 3 |
Uniprot ID | O75112 |
Protein Accession # | NP_009009.1 |
Molecular Weight | 77.1; 66.7; 35.6; 31.0 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "Goat Anti-ZASP / CYPHER / LDB3 Antibody (OAEB01548)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Dog|Human|Mouse|Porcine|Rat".
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How long will it take to receive "Goat Anti-ZASP / CYPHER / LDB3 Antibody (OAEB01548)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "Goat Anti-ZASP / CYPHER / LDB3 Antibody (OAEB01548)" provided in?
This item is provided in "Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "Goat Anti-ZASP / CYPHER / LDB3 Antibody (OAEB01548)"?
This target may also be called "cardiomyopathy, dilated 1C (autosomal dominant);CMD1C;CMH24;CMPD3;CYPHER;LDB3Z1;LDB3Z4;LIM domain-binding protein 3;LIM domain-binding protein 3 isoform;LVNC3;MFM4;ORACLE;PDLIM6;PDZ and LIM domain 6;protein cypher;ZASP;Z-band alternatively spliced PDZ-motif protein." in publications.
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What is the shipping cost for "Goat Anti-ZASP / CYPHER / LDB3 Antibody (OAEB01548)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "Goat Anti-ZASP / CYPHER / LDB3 Antibody (OAEB01548)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "Goat Anti-ZASP / CYPHER / LDB3 Antibody (OAEB01548)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "77.1; 66.7; 35.6; 31.0".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "Goat Anti-ZASP / CYPHER / LDB3 Antibody (OAEB01548)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "LDB3"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "LDB3"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "LDB3"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "LDB3"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "LDB3"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "LDB3"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.