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| Datasheets/Manuals | Printable datasheet for Goat Anti-TRIM35 / MAIR Antibody (OAEB00345) |
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| Predicted Species Reactivity | Human |
|---|---|
| Product Format | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Clonality | Polyclonal |
| Host | Goat |
| Application | WB |
| Reconstitution and Storage | Aliquot and store at -20C. Minimize freezing and thawing. |
| Immunogen | TRIM35 |
| Purification | Ammonium Sulfate Precipitated; Affinity Purified |
| Predicted Homology Based on Immunogen Sequence | Human: 100% |
| Peptide Sequence | ERSPDVSPGPSRS-C |
| Concentration | 0.5 mg/ml |
| Purity | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Application Info | Peptide ELISA: antibody detection limit dilution 1:32000. Western Blot: Approx. 25-30kDa band observed in human testis and human brain lysates at 1ug of primary, this band was successfully blocked by incubation with the immunising peptide. Please note that the band we observe is different from the predicted sizes of approx. 57kDa and 24kDa according to NP_055881and NP_741983. Currently we cannot find an explanation for this in the literature. |
| Reference | * Kimura F, Suzu S, Nakamura Y, Nakata Y, Yamada M, Kuwada N, Matsumura T, Yamashita T, Ikeda T, Sato K, Motoyoshi K.; Cloning and characterization of a novel RING-B-box-coiled-coil protein with apoptotic function.; J Biol Chem. 2003 Jul 4;278(27):25046-54.; Pubmed 12692137 |
| Gene Symbol | TRIM35 |
|---|---|
| Gene Full Name | tripartite motif containing 35 |
| Alias Symbols | E3 ubiquitin-protein ligase TRIM35;hemopoietic lineage switch protein 5;HLS5;MAIR;tripartite motif-containing protein 35. |
| NCBI Gene Id | 23087 |
| Protein Name | Tripartite motif-containing protein 35 |
| Uniprot ID | Q9UPQ4 |
| Protein Accession # | NP_741983.2 |
| Molecular Weight | 56.5 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "Goat Anti-TRIM35 / MAIR Antibody (OAEB00345)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human".
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How long will it take to receive "Goat Anti-TRIM35 / MAIR Antibody (OAEB00345)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "Goat Anti-TRIM35 / MAIR Antibody (OAEB00345)" provided in?
This item is provided in "Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "Goat Anti-TRIM35 / MAIR Antibody (OAEB00345)"?
This target may also be called "E3 ubiquitin-protein ligase TRIM35;hemopoietic lineage switch protein 5;HLS5;MAIR;tripartite motif-containing protein 35." in publications.
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What is the shipping cost for "Goat Anti-TRIM35 / MAIR Antibody (OAEB00345)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "Goat Anti-TRIM35 / MAIR Antibody (OAEB00345)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "Goat Anti-TRIM35 / MAIR Antibody (OAEB00345)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "56.5".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "Goat Anti-TRIM35 / MAIR Antibody (OAEB00345)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "TRIM35"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "TRIM35"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "TRIM35"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "TRIM35"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "TRIM35"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "TRIM35"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
