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Goat Anti-TCF3 / ITF1 Antibody (OAEB02356)

100 ug
$349.00
In Stock
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Gene Symbol:
TCF3
NCBI Gene Id:
6929
Protein Name:
Transcription factor E2-alpha
Swissprot Id:
P15923
Protein Accession #:
NP_003191.1
Replacement Item:
This antibody may replace item sc-12491 from Santa Cruz Biotechnology.
Host:
Goat
Clonality:
Polyclonal
Application:
WB, IHC
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express TCF3.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express TCF3.
Predicted Species Reactivity:
Dog, Human, Mouse, Pig, Rat
Predicted Homology Based on Immunogen Sequence:
Dog: 78%; Human: 100%; Mouse: 85%; Pig: 92%; Rat: 85%
Complete computational species homology data:
TCF3 Antibody (OAEB02356)
Peptide Sequence:
C-KAPRARTSPDEDED
Product Format:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Reconstitution and Storage:
Aliquot and store at -20C. Minimize freezing and thawing.
Datasheets/Manuals:
Printable datasheet for anti-TCF3 antibody - OAEB02356
Purity:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Application Info:
Peptide ELISA: antibody detection limit dilution 1:128000.
Western Blot: Approx 75kDa band observed in lysates of cell line Daudi (calculated MW of 67.6kDa according to NP_003191.1). Recommended concentration: 0.01-0.03ug/ml.
Immunohistochemistry: In paraffin embedded Human Kidney shows strong nuclear staining in DCT cells. Recommended concentration, 3-5ug/ml.
Additional Information:
Reactivity Info: Tested: Human; Expected: Human
Target Reference:
* Greenbaum S, Lazorchak AS, Zhuang Y.; Differential functions for the transcription factor E2A in positive and negative gene regulation in pre-B lymphocytes.; J Biol Chem. 2004 Oct 22;279(43):45028-35.; Pubmed 15310760

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