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Datasheets/Manuals | Printable datasheet for Goat Anti-TCF3 / ITF1 Antibody (OAEB02356) |
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Predicted Species Reactivity | Human |
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Product Format | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Clonality | Polyclonal |
Host | Goat |
Application | WB, IHC |
Additional Information | Reactivity Info: Tested: Human; Expected: Human |
Reconstitution and Storage | Aliquot and store at -20C. Minimize freezing and thawing. |
Immunogen | TCF3 |
Purification | Ammonium Sulfate Precipitated; Affinity Purified |
Predicted Homology Based on Immunogen Sequence | Dog: 78%; Human: 100%; Mouse: 85%; Pig: 92%; Rat: 85% |
Peptide Sequence | C-KAPRARTSPDEDED |
Concentration | 0.5 mg/ml |
Purity | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Application Info | Peptide ELISA: antibody detection limit dilution 1:128000. Western Blot: Approx 75kDa band observed in lysates of cell line Daudi (calculated MW of 67.6kDa according to NP_003191.1). Recommended concentration: 0.01-0.03ug/ml. Immunohistochemistry: In paraffin embedded Human Kidney shows strong nuclear staining in DCT cells. Recommended concentration, 3-5ug/ml. |
Reference | * Greenbaum S, Lazorchak AS, Zhuang Y.; Differential functions for the transcription factor E2A in positive and negative gene regulation in pre-B lymphocytes.; J Biol Chem. 2004 Oct 22;279(43):45028-35.; Pubmed 15310760 |
Gene Symbol | TCF3 |
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Gene Full Name | transcription factor 3 |
Alias Symbols | AGM8;bHLHb21;class B basic helix-loop-helix protein 21;E2A;E2A-HLF fusion transcript protein;E47;helix-loop-helix protein HE47;immunoglobulin transcription factor 1;ITF1;kappa-E2-binding factor;negative vitamin D response element-binding protein;NOL1-TCF3 fusion;p75;TCF-3;transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47);transcription factor 3 variant 3;transcription factor E2-alpha;transcription factor ITF-1;VDIR;VDR interacting repressor;vitamin D receptor-interacting repressor. |
NCBI Gene Id | 6929 |
Protein Name | Transcription factor E2-alpha |
Uniprot ID | P15923 |
Protein Accession # | NP_003191.1 |
Molecular Weight | 67.6 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "Goat Anti-TCF3 / ITF1 Antibody (OAEB02356)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human".
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How long will it take to receive "Goat Anti-TCF3 / ITF1 Antibody (OAEB02356)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "Goat Anti-TCF3 / ITF1 Antibody (OAEB02356)" provided in?
This item is provided in "Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "Goat Anti-TCF3 / ITF1 Antibody (OAEB02356)"?
This target may also be called "AGM8;bHLHb21;class B basic helix-loop-helix protein 21;E2A;E2A-HLF fusion transcript protein;E47;helix-loop-helix protein HE47;immunoglobulin transcription factor 1;ITF1;kappa-E2-binding factor;negative vitamin D response element-binding protein;NOL1-TCF3 fusion;p75;TCF-3;transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47);transcription factor 3 variant 3;transcription factor E2-alpha;transcription factor ITF-1;VDIR;VDR interacting repressor;vitamin D receptor-interacting repressor." in publications.
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What is the shipping cost for "Goat Anti-TCF3 / ITF1 Antibody (OAEB02356)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "Goat Anti-TCF3 / ITF1 Antibody (OAEB02356)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "Goat Anti-TCF3 / ITF1 Antibody (OAEB02356)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "67.6".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "Goat Anti-TCF3 / ITF1 Antibody (OAEB02356)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "TCF3"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "TCF3"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "TCF3"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "TCF3"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "TCF3"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "TCF3"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.