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| Datasheets/Manuals | Printable datasheet for Goat Anti-SPEN / SHARP Antibody (OAEB00566) |
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| Predicted Species Reactivity | Human |
|---|---|
| Product Format | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Clonality | Polyclonal |
| Host | Goat |
| Application | P-WB |
| Reconstitution and Storage | Aliquot and store at -20C. Minimize freezing and thawing. |
| Immunogen | SPEN |
| Purification | Ammonium Sulfate Precipitated; Affinity Purified |
| Predicted Homology Based on Immunogen Sequence | Human |
| Peptide Sequence | C-DSEGKMDDKKEDHKE |
| Concentration | 0.5 mg/ml |
| Application Info | P-ELISA: Antibody detection limit dilution 1:4000 WB: Not yet tested - the routinely used western blotting protocol does not allow detection of proteins as large as the calculated size of approx. 402kDa according to NP_055816.2. Therefore an optimal concentration cannot be recommended and the product is investigative grade. |
| Reference | * Hiriart E, Gruffat H, Buisson M, Mikaelian I, Keppler S, Meresse P, Mercher T, Bernard OA, Sergeant A, Manet E.; Interaction of the Epstein-Barr virus mRNA export factor EB2 with human Spen proteins SHARP, OTT1, and a novel member of the family, OTT3, links Spen proteins with splicing regulation and mRNA export.; J Biol Chem. 2005 Nov 4;280(44):36935-45. Epub 2005 Aug 29.; Pubmed 16129689 |
| Gene Symbol | SPEN |
|---|---|
| Gene Full Name | spen family transcriptional repressor |
| Alias Symbols | HIAA0929;MINT;Msx2 interacting nuclear target (MINT) homolog;msx2-interacting protein;nuclear receptor transcription cofactor;RATARS;RBM15C;SHARP;SMART/HDAC1-associated repressor protein;spen homolog, transcriptional regulator. |
| NCBI Gene Id | 23013 |
| Protein Name | Msx2-interacting protein |
| Uniprot ID | Q96T58 |
| Protein Accession # | NP_055816.2 |
| Molecular Weight | 402 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "Goat Anti-SPEN / SHARP Antibody (OAEB00566)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human".
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How long will it take to receive "Goat Anti-SPEN / SHARP Antibody (OAEB00566)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "Goat Anti-SPEN / SHARP Antibody (OAEB00566)" provided in?
This item is provided in "Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "Goat Anti-SPEN / SHARP Antibody (OAEB00566)"?
This target may also be called "HIAA0929;MINT;Msx2 interacting nuclear target (MINT) homolog;msx2-interacting protein;nuclear receptor transcription cofactor;RATARS;RBM15C;SHARP;SMART/HDAC1-associated repressor protein;spen homolog, transcriptional regulator." in publications.
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What is the shipping cost for "Goat Anti-SPEN / SHARP Antibody (OAEB00566)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "Goat Anti-SPEN / SHARP Antibody (OAEB00566)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "Goat Anti-SPEN / SHARP Antibody (OAEB00566)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "402".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "Goat Anti-SPEN / SHARP Antibody (OAEB00566)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "SPEN"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "SPEN"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "SPEN"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "SPEN"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "SPEN"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "SPEN"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.