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| Datasheets/Manuals | Printable datasheet for Goat Anti-Sirtuin 1 / SIRT1 (aa577-590) Antibody (OAEB01045) |
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| Predicted Species Reactivity | Human|Mouse |
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| Product Format | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Clonality | Polyclonal |
| Host | Goat |
| Application | IHC, WB |
| Additional Information | Epitope Info: internal region Immunogen: Peptide with sequence C-EKPQEVQTSRNVES, from the internal region of the protein sequence according to NP_036370.2; NP_001135970.1. |
| :: | Note: This antibody is expected to recognize both reported isoforms (NP_036370.2; NP_001135970.1). Reactivity Info: Tested: Human; Expected: Human, Mouse |
| Reconstitution and Storage | Aliquot and store at -20C. Minimize freezing and thawing. |
| Immunogen | SIRT1 |
| Purification | Ammonium Sulfate Precipitated; Affinity Purified |
| Predicted Homology Based on Immunogen Sequence | Guinea Pig: 86%; Human: 100%; Mouse: 100%; Pig: 78%; Rat: 85% |
| Peptide Sequence | C-EKPQEVQTSRNVES |
| Concentration | 0.5 mg/ml |
| Purity | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Application Info | Peptide ELISA: Antibody detection limit dilution 1:64000. Western Blot: Approx 120kDa band observed in lysates of cell line Jurkat (calculated MW of 81.7kDa according to NP_036370.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Cheng et al, Proc Natl Acad Sci U S A. 2003 Sep 16;100(19):10794-9.; PMID: 12960381). Recommended concentration: 0.05-0.2ug/ml. |
| Gene Symbol | Sirt1|SIRT1 |
|---|---|
| Gene Full Name | sirtuin 1 |
| Alias Symbols | AA673258;NAD-dependent protein deacetylase sirtuin-1;NAD-dependent protein deacylase sirtuin-1;regulatory protein SIR2 homolog 1;S;Si;Sir2;Sir2a;Sir2alpha;SIR2L1;SIR2-like protein 1;sirtuin 1 ((silent mating type information regulation 2, homolog) 1;sirtuin type 1. |
| NCBI Gene Id | 23411|93759 |
| Protein Name | NAD-dependent protein deacetylase sirtuin-1 |
| Uniprot ID | Q96EB6 |
| Protein Accession # | NP_036370.2 |
| Molecular Weight | 81.7; 50.5 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "Goat Anti-Sirtuin 1 / SIRT1 (aa577-590) Antibody (OAEB01045)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse".
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How long will it take to receive "Goat Anti-Sirtuin 1 / SIRT1 (aa577-590) Antibody (OAEB01045)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "Goat Anti-Sirtuin 1 / SIRT1 (aa577-590) Antibody (OAEB01045)" provided in?
This item is provided in "Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "Goat Anti-Sirtuin 1 / SIRT1 (aa577-590) Antibody (OAEB01045)"?
This target may also be called "AA673258;NAD-dependent protein deacetylase sirtuin-1;NAD-dependent protein deacylase sirtuin-1;regulatory protein SIR2 homolog 1;S;Si;Sir2;Sir2a;Sir2alpha;SIR2L1;SIR2-like protein 1;sirtuin 1 ((silent mating type information regulation 2, homolog) 1;sirtuin type 1." in publications.
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What is the shipping cost for "Goat Anti-Sirtuin 1 / SIRT1 (aa577-590) Antibody (OAEB01045)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "Goat Anti-Sirtuin 1 / SIRT1 (aa577-590) Antibody (OAEB01045)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "Goat Anti-Sirtuin 1 / SIRT1 (aa577-590) Antibody (OAEB01045)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "81.7; 50.5".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "Goat Anti-Sirtuin 1 / SIRT1 (aa577-590) Antibody (OAEB01045)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "Sirt1|SIRT1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "Sirt1|SIRT1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "Sirt1|SIRT1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "Sirt1|SIRT1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "Sirt1|SIRT1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "Sirt1|SIRT1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
