- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Printable datasheet for Goat Anti-SIAHBP1 / FIR Antibody (OAEB01786) |
---|
Predicted Species Reactivity | Bovine|Dog|Human|Mouse|Rat |
---|---|
Product Format | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Clonality | Polyclonal |
Host | Goat |
Application | WB |
Reconstitution and Storage | Aliquot and store at -20C. Minimize freezing and thawing. |
Immunogen | PUF60 |
Purification | Ammonium Sulfate Precipitated; Affinity Purified |
Predicted Homology Based on Immunogen Sequence | Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rat: 100%; Zebrafish: 100% |
Peptide Sequence | C-YDQERFDNSDLSA |
Concentration | 0.5 mg/ml |
Purity | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Application Info | Peptide ELISA: antibody detection limit dilution 1:32000. Western Blot: Approx 60kDa band observed in lysate of human epidermoid carcinoma A431 (calculated MW of 59.9kDa (isoform A) according to NP_510965.1 and 58.2kDa (isoform B) according to NP_055096.2). Recommended concentration: 1-3ug/ml. |
Reference | * Corsini L, Hothorn M, Stier G, Rybin V, Scheffzek K, Gibson TJ, Sattler M.; Dimerization and protein binding specificity of the U2AF homology motif of the splicing factor Puf60.; J Biol Chem. 2009 Jan 2;284(1):630-9.; Pubmed 18974054 * Liu J, He L, Collins I, Ge H, Libutti D, Li J, Egly JM, Levens D.; The FBP interacting repressor targets TFIIH to inhibit activated transcription.; Mol Cell. 2000 Feb;5(2):331-41.; Pubmed 10882074 |
Gene Symbol | Puf60|PUF60 |
---|---|
Gene Full Name | poly(U) binding splicing factor 60|poly-U binding splicing factor 60 |
Alias Symbols | 2410104I19Rik;2810454F19Rik;60 kDa poly(U)-binding-splicing factor;FBP interacting repressor;FIR;fuse-binding protein-interacting repressor;poly(U) binding splicing factor 60KDa;poly(U)-binding-splicing factor PUF60;pyrimidine tract binding splicing factor;Ro ribonucleoprotein-binding protein 1;RoBPI;Siah binding protein 1;SIAHBP1;VRJS. |
NCBI Gene Id | 22827|67959 |
Protein Name | Poly(U)-binding-splicing factor PUF60 |
Uniprot ID | Q9UHX1 |
Protein Accession # | NP_510965.1 |
Molecular Weight | 59.9; 58.2; 55.7 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
-
What is the species homology for "Goat Anti-SIAHBP1 / FIR Antibody (OAEB01786)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Bovine|Dog|Human|Mouse|Rat".
-
How long will it take to receive "Goat Anti-SIAHBP1 / FIR Antibody (OAEB01786)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
-
What buffer format is "Goat Anti-SIAHBP1 / FIR Antibody (OAEB01786)" provided in?
This item is provided in "Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "Goat Anti-SIAHBP1 / FIR Antibody (OAEB01786)"?
This target may also be called "2410104I19Rik;2810454F19Rik;60 kDa poly(U)-binding-splicing factor;FBP interacting repressor;FIR;fuse-binding protein-interacting repressor;poly(U) binding splicing factor 60KDa;poly(U)-binding-splicing factor PUF60;pyrimidine tract binding splicing factor;Ro ribonucleoprotein-binding protein 1;RoBPI;Siah binding protein 1;SIAHBP1;VRJS." in publications.
-
What is the shipping cost for "Goat Anti-SIAHBP1 / FIR Antibody (OAEB01786)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
-
What is the guarantee for "Goat Anti-SIAHBP1 / FIR Antibody (OAEB01786)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
-
Can I get bulk pricing for "Goat Anti-SIAHBP1 / FIR Antibody (OAEB01786)"?
You can get bulk pricing for this item by going here.
-
What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "59.9; 58.2; 55.7".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "Goat Anti-SIAHBP1 / FIR Antibody (OAEB01786)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
-
What are positive controls for "Puf60|PUF60"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
-
What are negative controls for "Puf60|PUF60"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
-
What other proteins interact with "Puf60|PUF60"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
-
What biological processes are associated with "Puf60|PUF60"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
-
What cellular components are associated with "Puf60|PUF60"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
-
What protein functions are associated with "Puf60|PUF60"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.