Goat Anti-S3-12 / KIAA1881 Antibody (OAEB00301)

Catalog No: OAEB00301 (Formerly GWB-B54033)

Size:100UG

OAEB00301 (3.8ug/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: IHC
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: KIAA1881
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human: 100%
• Peptide Sequence: C-RTTDNGGEQTALSPQE
• Concentration: 0.5 mg/ml
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Application Info: Peptide ELISA: antibody detection limit dilution 1:2000.
  Western Blot: Preliminary experiments gave bands at approx 45kDa and 50kDa in Human Adipose lysates after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 134kDa according to NP_001073869.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
• Reference: * Wolins NE, Brasaemle DL, Bickel PE.; A proposed model of fat packaging by exchangeable lipid droplet proteins.; FEBS Lett. 2006 Oct 9;580(23):5484-91. Epub 2006 Sep 1. Review.; Pubmed 16962104

Target Info

• Gene Symbol: PLIN4
• Gene Full Name: perilipin 4
• Alias Symbols: adipocyte protein S3-12;KIAA1881;perilipin-4;S3-12.
• NCBI Gene Id: 729359
• Protein Name: Perilipin-4
• Uniprot ID: Q96Q06
• Protein Accession #: NP_001073869.1
• Molecular Weight: 134