Goat Anti-RACGAP1 / MgcRacGAP Antibody (OAEB02427)

Catalog No: OAEB02427 (Formerly GWB-C94159)

Size:100UG

OAEB02427 (1ug/ml) staining of K562 (A) and Jurkat (B) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

OAEB02427 (1ug/ml) staining of paraffin embedded Human Testis. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining. Data obtained with a previous batch.

Product Info

• Predicted Species Reactivity: Bovine|Dog|Human
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: WB, IHC
• Additional Information: Reactivity Info: Tested: Human; Expected: Human, Dog, Cow
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: RACGAP1
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Horse: 100%; Human: 100%; Pig: 100%; Rabbit: 92%; Zebrafish: 91%
• Peptide Sequence: C-GRQGNFFASPMLK
• Concentration: 0.5 mg/ml
• Specificity: Reported variants represent identical protein: NP_037409.2, NP_001119576.1 and NP_001119575.1.
• Application Info: Peptide ELISA: antibody detection limit dilution 1:32000.
  Western Blot: Approx 75kDa band observed in lysates of cell line K562 and Jurkat (calculated MW of 71.0kDa according to NP_037409.2). Recommended concentration: 0.3-1ug/ml.
  Immunohistochemistry: In paraffin embedded Human Testis, shows nuclear staining of many cells. Recommended concentration, 1ug/ml.
• Reference: * Seguin L, Liot C, Mzali R, Harada R, Siret A, Nepveu A, Bertoglio J.; CUX1 and E2F1 regulate coordinated expression of the mitotic complex genes Ect2, MgcRacGAP, and MKLP1 in S phase.; Mol Cell Biol. 2009 Jan;29(2):570-81.; Pubmed 19015243
  * Grewal S, Carver JG, Ridley AJ, Mardon HJ.; Implantation of the human embryo requires Rac1-dependent endometrial stromal cell migration.; Proc Natl Acad Sci U S A. 2008 Oct 21;105(42):16189-94.; Pubmed 18838676
  * Toure A, Mzali R, Liot C, Seguin L, Morin L, Crouin C, Chen-Yang I, Tsay YG, Dorseuil O, Gacon G, Bertoglio J.; Phosphoregulation of MgcRacGAP in mitosis involves Aurora B and Cdk1 protein kinases and the PP2A phosphatase.; FEBS Lett. 2008 Apr 9;582(8):1182-8.; Pubmed 18201571
  * Zhao WM, Seki A, Fang G.; Cep55, a microtubule-bundling protein, associateswith centralspindlin to control the midbody integrity and cell abscission during cytokinesis.; Mol Biol Cell. 2006 Sep;17(9):3881-96.; Pubmed 16790497
  * Nishimura Y, Yonemura S.; Centralspindlin regulates ECT2 and RhoA accumulation at the equatorial cortex during cytokinesis.; J Cell Sci. 2006 Jan 1;119(Pt 1):104-14. Epub 2005 Dec 13.; Pubmed 16352658
  * Reibel L, Dorseuil O, Stancou R, Bertoglio J, Gacon G.; A hemopoietic specific gene encoding a small GTP binding protein is overexpressed during T cell activation.; Biochem Biophys Res Commun. 1991 Mar 15;175(2):451-8.; Pubmed 1902092

Target Info

• Gene Symbol: RACGAP1
• Gene Full Name: Rac GTPase activating protein 1
• Alias Symbols: CYK4;HsCYK-4;ID-GAP;male germ cell RacGap;MgcRacGAP;protein CYK4 homolog;rac GTPase-activating protein 1.
• NCBI Gene Id: 29127
• Protein Name: Rac GTPase-activating protein 1
• Uniprot ID: Q9H0H5
• Protein Accession #: NP_037409.2
• Molecular Weight: 71.0; 63.9; 72.3; 61.9