Goat Anti-Rabbit IgG Antibody: HRP (ASP00001)

Catalog No: ASP00001

Size:1.6 mg

Product Info

• Predicted Species Reactivity: Rabbit
• Product Format: Lyophilized 0.01M Sodium Phosphate, 0.25M NaCl (pH 7.6), 15 mg/ml BSA (IgG & Protease free)
• Isotype: IgG
• Host: Goat
• Conjugation: HRP: Horseradish Peroxidase
• Application: IHC, ICC, ELISA, WB (chromogenic substrates), WB (ECL substrates)
• Reconstitution and Storage: Store freeze-dried powder at 2-8°C. When ready to use, rehydrate with 2.0 mL dH₂O and centrifuge if not clear. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid. Prepare working dilution fresh each day. For extended storage after rehydration, aliquot and freeze at -70°C or below. Avoid repeated freezing and thawing. Alternatively, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid. Note that adding glycerol reduces the stated protein concentration and dilution range by oen-half. Expiration date is one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.
• Purification: The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
• Concentration: 0.8 mg/ml (prior to lyophilization)
• Specificity: Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule rabbit IgG. It also reacts with the light chains of other rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody may cross-react with immunoglobulins from other species.
• Warning: Use of sodium azide as a preservative will substantially inhibit the enzymatic activity of horseradish peroxidase. Confirm that glycerol is ACS grade or better before adding to this product. Lower grades of glycerol may seriously inhibit peroxidase enzyme activity.
• Dilution: Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
• Application Info: IHC/ICC: 1:500~5000
  ELISA/WB (chromogenic substrates): 1:5000~100000
  WB (ECL substrates): 1:10000~200000
• Publications:

  Chung, W.-G., Sandoval, M. A., Sloat, B. R., Lansakara-P, D. S. P. & Cui, Z. Stearoyl gemcitabine nanoparticles overcome resistance related to the over-expression of ribonucleotide reductase subunit M1. J. Control. Release 157, 132-40 (2012). 21851843

  Differential Effects of Resveratrol on the Expression of Brain-Derived Neurotrophic Factor Transcripts and Protein in the Hippocampus of Rat Brain. Iran J Med Sci. 42, 32-39 (2017). 28293048

  Divergent Hemogen genes of teleosts and mammals share conserved roles in erythropoiesis: analysis using transgenic and mutant zebrafish. Biol Open. 7, (2018). 30097520

  Effects of Ethanol on the Expression Level of Various BDNF mRNA Isoforms and Their Encoded Protein in the Hippocampus of Adult and Embryonic Rats. Int J Mol Sci. 16, 30422-37 (2015). 26703578

  Silencing of ribonucleotide reductase subunit M1 potentiates the antitumor activity of gemcitabine in resistant cancer cells. Cancer Biol Ther. 13, 908-14 (2012) 22785206

• Description:
  View product citations for Goat Anti-Rabbit IgG Antibody: HRP (ASP00001) on CiteAb

Target Info

• Description of Target: They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.
• Molecular Weight: ~160 kDa