Goat Anti-RAB11FIP1 / RCP Antibody (OAEB01053)

Catalog No: OAEB01053 (Formerly GWB-69783D)

Size:100UG

OAEB01053 (0.2ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: WB
• Additional Information: Epitope Info: internal region
  Immunogen: Peptide with sequence C-EATKEAKESKKPE, from the internal region of the protein sequence according to NP_079427.3; NP_001002814.1.
  Note: This antibody is expected to recognise isoform 1 (NP_079427.3) and isoform 3 (NP_001002814.1).
  Reactivity Info: Tested: Human; Expected: Human
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: RAB11FIP1
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human: 100%
• Peptide Sequence: C-EATKEAKESKKPE
• Concentration: 0.5 mg/ml
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Application Info: Peptide ELISA: Antibody detection limit dilution 1:64000.
  Western Blot: Approx 80kDa band observed in lysates of cell line HeLa (calculated MW of 71.0kDa according to NP_079427.3). The observed molecular weight of 80kDa corresponds to earlier findings in literature with different antibodies (Lindsay et al, J Biol Chem. 2002 Apr 5;277(14):12190-9. PMID 11786538.). Recommended concentration: 0.1-0.3ug/ml.
• Reference: Garcia MJ, Pole JC, Chin SF, Teschendorff A, Naderi A, Ozdag H, Vias M, Kranjac T, Subkhankulova T, Paish C, Ellis I, Brenton JD, Edwards PA, Caldas C.
  A 1 Mb minimal amplicon at 8p11-12 in breast cancer identifies new candidate oncogenes.
  Oncogene. 2005 Aug 4;24(33):5235-45. PMID: 15897872

Target Info

• Gene Symbol: RAB11FIP1
• Gene Full Name: RAB11 family interacting protein 1
• Alias Symbols: NOEL1A;Rab effector protein;RAB11 coupling protein;RAB11 family interacting protein 1 (class I);rab11 family-interacting protein 1;rab11-FIP1;Rab-interacting recycling protein;RCP.
• NCBI Gene Id: 80223
• Protein Name: RAB11-family interacting protein 1C EMBL ABB43161.1
• Uniprot ID: Q307T1
• Protein Accession #: NP_079427.3
• Molecular Weight: 71.0; 137