Goat Anti-PCK1 / PEPCKC (internal) Antibody (OAEB00932)

Catalog No: OAEB00932 (Formerly GWB-405565)

Size:100UG

OAEB00932 (2ug/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Info

• Predicted Species Reactivity: Bovine|Dog|Human|Mouse|Porcine|Rat
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: IHC
• Additional Information: Reactivity Info: Tested: Human; Expected: Human, Mouse, Rat, Dog
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: PCK1
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 90%; Human: 100%; Mouse: 91%; Pig: 90%; Rabbit: 100%
• Peptide Sequence: C-HVNWFRKDKEGK
• Concentration: 0.5 mg/ml
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Application Info: Peptide ELISA: antibody detection limit dilution 1:8000.
  Western Blot: Preliminary experiments gave bands at approx 70kDa, 45kDa and 28kDa in Human Adipose, Kidney and Liver lysates after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the additional bands we observe given the calculated size of 69.2 kDa according to NP_002582.2. All three detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
• Reference: * Yang Q, Graham TE, Mody N, Preitner F, Peroni OD, Zabolotny JM, Kotani K, Quadro L, Kahn BB.; Serum retinol binding protein 4 contributes to insulin resistance in obesity and type 2 diabetes.; Nature. 2005 Jul 21;436(7049):356-62.; Pubmed 16034410

Target Info

• Gene Symbol: PCK1
• Gene Full Name: phosphoenolpyruvate carboxykinase 1
• Alias Symbols: PCKDC;PEP carboxykinase;PEPCK1;PEPCKC;PEPCK-C;phosphoenolpyruvate carboxykinase 1 (soluble);phosphoenolpyruvate carboxykinase, cytosolic;phosphoenolpyruvate carboxykinase, cytosolic [GTP];phosphoenolpyruvate carboxylase;phosphopyruvate carboxylase;serine-protein kinase PCK1.
• NCBI Gene Id: 5105
• Protein Name: Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
• Uniprot ID: P35558
• Protein Accession #: NP_002582.3
• Molecular Weight: 69.2