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Goat Anti-OCT4 / POU5F1 (mouse) Antibody (OAEB00964)

100 ug
$349.00
In Stock
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Gene Symbol:
POU5F1
NCBI Gene Id:
18999
Protein Name:
POU domain, class 5, transcription factor 1
Swissprot Id:
P20263
Protein Accession #:
NP_038661.2
Alias Symbols:
Pou5f1, POU domain, class 5, transcription factor 1, Oct-3, Oct-3/4, Oct-4, Oct3, Oct3/4, Oct4, Otf-3, Otf-4, Otf3, Otf3-rs7, Otf3g, Otf4
Replacement Item:
This antibody may replace item sc-101534 from Santa Cruz Biotechnology.
Host:
Goat
Clonality:
Polyclonal
Application:
WB
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express POU5F1.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express POU5F1.
Predicted Species Reactivity:
Rat
Predicted Homology Based on Immunogen Sequence:
Rat: 91%
Complete computational species homology data:
POU5F1 Antibody (OAEB00964)
Peptide Sequence:
C-DRPNAVKLEKVEP
Product Format:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Reconstitution and Storage:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Concentration:
100ug specific antibody in 200ul
Datasheets/Manuals:
Printable datasheet for anti-POU5F1 antibody - OAEB00964
Purity:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Application Info:
Peptide ELISA: Antibody detection limit dilution 1:16000.
Western Blot: Approx 40kDa band observed in lysates of cell line NIH3T3 (calculated MW of 38.2kDa according to NP_038661.2). Recommended concentration: 1-3ug/ml.
Additional Information:
Epitope Info: Internal region
Immunogen: Peptide with sequence C-DRPNAVKLEKVEP, from the internal region of the protein sequence according to NP_038661.2.
:::
Reactivity Info: Tested: Mouse; Expected: Mouse, Rat
Target Reference:
Liu TM, Wu YN, Guo XM, Hui JH, Lee EH, Lim B, ; Effects of ectopic Nanog and Oct4 overexpression on mesenchymal stem cells.; Stem cells and development 2009 Sep 18 (7): 1013-22.; PMID: 19102659

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