Size:1 mg
$245.00
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SKU
OASB01625
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More Information
Tested Species Reactivity Mouse
Clonality Polyclonal
Isotype IgG
Host Goat
Application FC, IHC-F, IHC-P, ICC, WB, ELISPOT
Additional Information Description: Pooled antisera from goats hyperimmunized with mouse IgG3; purified by affinity chromatography on mouse IgG3 covalently linked to agarose; reacts with the heavy chain of mouse IgG3; cross-adsorbed against mouse IgG1, IgG2a, IgG2b, IgM, and IgA / human immunoglobulins and pooled sera for minimal reactivity
Reconstitution and Storage Store at 2-8C
Replacement Item This antibody may replace item sc-34663 from Santa Cruz Biotechnology.
Immunogen Mouse IgG3
Purification Affinity chromatography on mouse IgG3 covalently linked to agarose.
Concentration 1.0 mg/mL
Datasheets/Manuals Printable datasheet for anti-IGHG3 (OASB01625) antibody
Specificity IgG3
Characterization To insure lot- to- lot consistency, each batch of product is tested by ELISA, Fluorescent ELISA, and/or flowcytometry for conformance to characteristics of a standard reference reagent.
Warning Reagents contain sodium azide which is very toxic if ingested or inhaled. Avoid contact with skin, eyes, orclothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copiousamounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxichydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water before discardingto avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Dilution Immunofluorescence:
ELISA:
FITC, TRITC, TXRD BIOT, and CY5 conjugates
R-PE, SPRD, R-PE- CY7, and APC-CY7 conjugates
AP conjugate
HRP conjugate
BIOT conjugate
<= 1 ug/106 cells
<= 0.1 ug/106 cells
1:2,000-1:4,000
1:4,000-1:8,000
1:5,000-1:20,000
Application Info Indirect immunostaining in conjunction with mouse IgG3 monoclonal antibodies, Enzyme-Linked-Immunosorbent-Assay (ELISA), Immunoblotting
Other Applications Data Since applications vary, you should determine the optimum working dilutionof the product that is appropriate for your specific need.
Other Applications Image 1 Data Amount Used: u1 ug/106 cells Human peripheral blood mononuclear cells were labeled with either mouse anti-human CD3 (IgG1) (A), anti-human CD5 (IgG2a) (B), anti-human CD22 (IgG2b) (C), anti-human IgD (IgG3) (D) or anti-human CD57 (IgM) (E). After
Storage - The purified (UNLB) antibody is supplied as 1.0 mg purified immunoglobulin in 1.0 mL of 100 mM boratebuffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C
- The fluorescein (FITC), rhodamine (TRITC), Texas Red (TXRD), and Cyanine 5 (CY 5) conjugates aresupplied as 1.0 mg in 1.0 mL PBS/NaN3. Store at 2- 8 C
- The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mMMgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2- 8 C or long- term at - 20 C
- The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50%PBS, pH 7.4. No preservative added. Store at 2- 8 C or long- term at - 20 C
- The biotin (BIOT) conjugate is supplied as 1.0 mg in 2.0 mL PBS/NaN3. Store at 2- 8 C
- The R- phycoerythrin (R- PE) conjugate is supplied as 0.5 mg in 1.0 mL or 0.25 mg in 0.5 mL of PBS/NaN3and a stabilizing agent. Store at 2- 8 C. Do not freeze!
- The Spectral Red (SPRD) conjugate is supplied as 0.25 mg in 0.5 mL of PBS/NaN3 and a stabilizingagent. Store at 2- 8 C. Do not freeze!
- R- phycoerythrin- Cyanine 7 (R- PE- CY 7) and Allophycocyanin- Cyanine 7 (APC- CY 7) conjugates aresupplied as 0.25 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2- 8 C. Do not freeze!
- Protect fluorochrome- conjugated forms from light. Reagents are stable for the period shown on the label ifstored as directed.
Cross Absorption Mouse IgM, IgG1, IgG2a, IgG2b and IgA, pooled human sera and purified humanparaproteins.
Gene Symbol IGHG3
Official Gene Full Name Ig gamma-3 chain C region
Alias Symbols IgG3
NCBI Gene Id 380795
Swissprot Id P03987
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express IGHG3
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express IGHG3
  1. What is the species homology for "Mouse IgG3, Human ads Antibody (OASB01625)"?

    The tested species reactivity for this item is "Mouse". This antibody is predicted to have homology to "".

  2. How long will it take to receive "Mouse IgG3, Human ads Antibody (OASB01625)"?

    This item is available "Domestic: within 1 week delivery | International: 1 week".

  3. What buffer format is "Mouse IgG3, Human ads Antibody (OASB01625)" provided in?

    This item is provided in "".
    Additional format options may be available. For more information please contact info@avivasysbio.com.

  4. What are other names for "Mouse IgG3, Human ads Antibody (OASB01625)"?

    This target may also be called "IgG3" in publications.

  5. What is the shipping cost for "Mouse IgG3, Human ads Antibody (OASB01625)"?

    The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.

  6. What is the guarantee for "Mouse IgG3, Human ads Antibody (OASB01625)"?

    All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.

  7. Can I get bulk pricing for "Mouse IgG3, Human ads Antibody (OASB01625)"?

    You can get bulk pricing for this item by going here.

  8. What is the molecular weight of the protein?

    The molecular weight reported by Uniprot for this item is "".
    Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.

  9. What protocols are available for "Mouse IgG3, Human ads Antibody (OASB01625)"?

    We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data tab on the product page.

  10. What are positive controls for "IGHG3"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  11. What are negative controls for "IGHG3"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  12. What other proteins interact with "IGHG3"?

    This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.

  13. What biological processes are associated with "IGHG3"?

    This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.

  14. What cellular components are associated with "IGHG3"?

    This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.

  15. What protein functions are associated with "IGHG3"?

    This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.

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