Size:1 ml
$205.00
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OASB01607
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Mouse IgG2b, Human ads Antibody : AP (OASB01607)

Catalog#: OASB01607
Domestic: within 1 week delivery | International: 1 week
More Information
Tested Species ReactivityMouse
ClonalityPolyclonal
IsotypeIgG
HostGoat
ConjugationAP: Alkaline Phosphatase
ApplicationIHC-F, IHC-P, ICC, WB, ELISPOT
Additional InformationDescription: Pooled antisera from goats hyperimmunized with mouse IgG2b; purified by affinity chromatography on mouse IgG2b covalently linked to agarose; reacts with the heavy chain of mouse IgG2b; cross-adsorbed against mouse IgG1, IgG2b, IgG3, IgM, and IgA / human, bovine, and horse serum proteins (SP) for minimal reactivity
Reconstitution and StorageStore at 2-8C
ImmunogenMouse IgG2b
PurificationAffinity chromatography on mouse IgG2b covalently linked to agarose
ConcentrationLot specific
Datasheets/ManualsPrintable datasheet for anti-Igh-3 (OASB01607) antibody
SpecificityIgG2b
CharacterizationTo insure lot- to- lot consistency, each batch of product is tested by ELISA, FLISA, and/or flow cytometry forconformance to characteristics of a standard reference reagent.
WarningReagents contain sodium azide which is very toxic if ingested or inhaled. Avoid contact with skin, eyes, orclothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copiousamounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxichydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water before discardingto avoid accumulation of potentially explosive deposits in lead or copper plumbing.
DilutionImmunofluorescence:
FLISA:
ELISA:
FITC and BIOT conjugates
R-PE, APC, R-PE-CY7, APC-CY7, CY5 and
SPRD conjugates
TRITC and TXRD conjugates
AP conjugate
HRP conjugate
BIOT conjugate
<= 1 ug/106 cells
<= 0.1 ug/106 cells
1:100-1:400
1:2,000-1:4,000
1:4,000-1:8,000
1:5,000-1:20,000
Application InfoIndirect immunostaining in conjunction with mouse IgG2b monoclonal antibodies, Enzyme-Linked-Immunosorbent-Assay (ELISA), Fluorescent-Linked-Immunosorbent-Assay (FLISA), Immunoblotting
Other Applications DataSince applications vary, you should determine the optimum working dilution ofthe product that is appropriate for your specific need.
Other Applications Image 1 Data Amount Used: ≤1 ug/106 cells Human peripheral blood mononuclear cells were labeled with either mouse anti-human CD3 (IgG1) (A), anti-human CD5 (IgG2a) (B), anti-human CD22 (IgG2b) (C), anti-human IgD (IgG3) (D) or anti-human CD57 (IgM) (E). After
Storage- The purified (UNLB) antibody is supplied as 1.0 mg purified immunoglobulin in 1.0 mL of 100 mM boratebuffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C
- The fluorescein (FITC), rhodamine (TRITC), Texas Red (TXRD), and Cyanine 5 (CY 5) conjugates aresupplied as 1.0 mg in 1.0 mL PBS/NaN3. Store at 2- 8 C
- The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mMMgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2- 8 C or long- term at - 20 C
- The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50%PBS, pH 7.4. No preservative added. Store at 2- 8 C or long- term at - 20 C
- The biotin (BIOT) conjugate is supplied as 1.0 mg in 2.0 mL PBS/NaN3. Store at 2- 8 C
- The R- phycoerythrin (R- PE) conjugate is supplied as 0.5 mg in 1.0 mL or 0.25 mg in 0.5 mL of PBS/NaN3and a stabilizing agent. Store at 2- 8 C. Do not freeze!
- The allophycocyanin (APC) conjugate is supplied as 0.5 mg in 1.0 mL or 0.25 mg in 0.5 mL of PBS/NaN3and a stabilizing agent. Store at 2- 8 C. Do not freeze!
- The Spectral Red (SPRD), R- phycoerythrin- Cyanine 7 (R- PE- CY 7), and Allophycocyanin- Cyanine 7(APC- CY 7) conjugates are supplied as 0.25 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Storeat 2- 8 C. Do not freeze!
- Protect fluorochrome- conjugated forms from light. Reagents are stable for the period shown on the label ifstored as directed.
Cross AbsorptionMouse IgM, IgG1, IgG2a, IgG3 and IgA, pooled human sera and purified humanparaproteins.
Gene SymbolIGHG2B
Official Gene Full NameIg gamma-2B chain C region
Alias SymbolsIgG2b, Igh-3, gamma2b
NCBI Gene Id16016
Swissprot IdP01867
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express IGHG2B
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express IGHG2B
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