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Goat Anti-Mouse IgG2a, Human ads Antibody : HRP (OASB01588)

1 ml
$180.00
In Stock
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Tested Species Reactivity:
Mouse
Product Format:
Liquid. 50% Glycerol/50% Phosphate buffered saline, pH 7.4
Clonality:
Polyclonal
Isotype:
IgG
Host:
Goat
Conjugation:
HRP: Horseradish Peroxidase
Application:
ELISA, IHC-F, IHC-P, ICC, WB, ELISPOT
Additional Information:
Description: Pooled antisera from goats hyperimmunized with mouse IgG2a; purified by affinity chromatography on mouse IgG2a covalently linked to agarose; reacts with the heavy chain of mouse IgG2a; cross-adsorbed against mouse IgG1, IgG2b, IgG3, IgM, and IgA / human immunoglobulins and pooled sera for minimal reactivity
Reconstitution and Storage:
Store at 2-8C
Gene Symbol:
IGHG2A
Official Gene Full Name:
Ig gamma-2A chain C region
NCBI Gene Id:
380793
Swissprot Id:
P01863, P01864, P01865
Alias Symbols:
Igh-1, Igh-1a, 1810060O09Rik
Purification:
Affinity chromatography on mouse IgG2a covalently linked to agarose.
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express IGHG2A.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express IGHG2A.
Immunogen:
Mouse IgG2a
Concentration:
Lot specific
Datasheets/Manuals:
Printable datasheet for anti-Igg-2a (OASB01588) antibody
Specificity:
IgG2a
Characterization:
To insure lot- to- lot consistency, each batch of product is tested by ELISA, FLISA, and/or flow cytometry forconformance with characteristics of a standard reference reagent.
Warning:
Reagents contain sodium azide which is very toxic if ingested or inhaled. Avoid contact with skin, eyes, orclothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copiousamounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxichydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water before discardingto avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Dilution:
Immunofluorescence:
FLISA:
ELISA:
FITC and BIOT conjugates
R-PE, APC, SPRD, R-PE-TXRD, CY5, R-PE-CY7,
and APC-CY7 conjugates
TRITC and TXRD conjugates
AP conjugate
HRP conjugate
BIOT conjugate
<= 1 ug/106 cells
<= 0.1 ug/106 cells
1:100-1:400
1:2,000-1:4,000
1:4,000-1:8,000
1:5,000-1:20,000
Application Info:
Indirect immunostaining in conjunction with mouse IgG2a monoclonal antibodies, Enzyme-Linked-Immunosorbent-Assay (ELISA), Fluorescent-Linked-Immunosorbent-Assay (FLISA), Immunoblotting
Other Applications Data:
Since applications vary, you should determine the optimum working dilutionof the product that is appropriate for your specific need.
Other Applications Image 1 Data:
Amount Used: ≤1 ug/106 cells Human peripheral blood mononuclear cells were labeled with either mouse anti-human CD3 (IgG1), anti-human CD5 (IgG2a), anti-human CD22 (IgG2b), anti-human IgD (IgG3), or anti-human CD57 (IgM). After washing the cells
Storage:
- The purified (UNLB) antibody is supplied as 1.0 mg purified immunoglobulin in 1.0 mL of 100 mM boratebuffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C
- The fluorescein (FITC), rhodamine (TRITC), Texas Red (TXRD), and Cyanine 5 (CY 5) conjugates aresupplied as 1.0 mg in 1.0 mL PBS/NaN3. Store at 2- 8 C
- The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mMMgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2- 8 C or long- term at - 20 C
- The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50%PBS, pH 7.4. No preservative added. Store at 2- 8 C or long- term at - 20 C
- The biotin (BIOT) conjugate is supplied as 1.0 mg in 2.0 mL PBS/NaN3. Store at 2- 8 C
- The R- phycoerythrin (R- PE) conjugate is supplied as 0.5 mg in 1.0 mL or 0.25 mg in 0.5 mL of PBS/NaN3and a stabilizing agent. Store at 2- 8 C. Do not freeze!
- The allophycocyanin (APC) conjugate is supplied as 0.5 mg in 1.0 mL or 0.25 mg in 0.5 mL of PBS/NaN3and a stabilizing agent. Store at 2- 8 C. Do not freeze!
- The Spectral Red (SPRD), R- phycoerythrin - Texas Red (R- PE- TXRD), R- phycoerythrin- Cyanine 7(R- PE- CY 7), and Allophycocyanin- Cyanine 7 (APC- CY 7) conjugates are supplied as 0.25 mg in 1.0mL of PBS/NaN3 and a stabilizing agent. Store at 2- 8 C. Do not freeze!
- Protect fluorochrome- conjugated forms from light. Reagents are stable for the period shown on the label ifstored as directed.
Cross Absorption:
Mouse IgM, IgG1, IgG2b, IgG3 and IgA, pooled human sera and purified humanparaproteins.

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