Goat Anti-MEL18 / PCGF2 Antibody (OAEB01334)

Catalog No: OAEB01334 (Formerly GWB-B7E205)

Size:100UG

OAEB01334 staining (1ug/ml) of Human Lung lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Product Info

• Predicted Species Reactivity: Bovine|Dog|Human
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: WB
• Additional Information: Reactivity Info: Tested: Human; Expected: Human, Dog, Cow
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: PCGF2
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Human: 100%; Mouse: 90%; Rabbit: 100%; Rat: 90%
• Peptide Sequence: C-KMTVNGAPVPPLT
• Concentration: 0.5 mg/ml
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Application Info: Peptide ELISA: antibody detection limit dilution 1:16000.
  Western Blot: Approx 40 kDa band observed in Human Lung lysates (calculated MW of 37.8kDa according to NP_009075.1). Recommended for use at 1-2ug/ml.
• Reference: * Endoh M, Endo TA, Endoh T, Fujimura Y, Ohara O, Toyoda T, Otte AP, Okano M, Brockdorff N, Vidal M, Koseki H.; Polycomb group proteins Ring1A/B are functionally linked to the core transcriptional regulatory circuitry to maintain ES cell identity.; Development. 2008 Apr;135(8):1513-24.; Pubmed 18339675
  * Miki J, Fujimura Y, Koseki H, Kamijo T.; Polycomb complexes regulate cellular senescence by repression of ARF in cooperation with E2F3.; Genes Cells. 2007 Dec;12(12):1371-82.; Pubmed 18076574
  * Elderkin S, Maertens GN, Endoh M, Mallery DL, Morrice N, Koseki H, Peters G, Brockdorff N, Hiom K.; A phosphorylated form of Mel-18 targets the Ring1B histone H2A ubiquitin ligase to chromatin.; Mol Cell. 2007 Oct 12;28(1):107-20.; Pubmed 17936708
  * Xi S, Zhu H, Xu H, Schmidtmann A, Geiman TM, Muegge K.; Lsh controls Hox gene silencing during development.; Proc Natl Acad Sci U S A. 2007 Sep 4;104(36):14366-71.; Pubmed 17726103
  * Ishida A, Asano H, Hasegawa M, Koseki H, Ono T, Yoshida MC, Taniguchi M, Kanno M.; Cloning and chromosome mapping of the human Mel-18 gene which encodes a DNA-binding protein with a new 'RING-finger' motif.; Gene. 1993 Jul 30;129(2):249-55.; Pubmed 8325509

Target Info

• Gene Symbol: PCGF2
• Gene Full Name: polycomb group ring finger 2
• Alias Symbols: DNA-binding protein Mel-18;MEL-18;polycomb group RING finger protein 2;ring finger protein 110;RNF110;TPFS;zinc finger protein 144;ZNF144.
• NCBI Gene Id: 7703
• Protein Name: Polycomb group RING finger protein 2
• Uniprot ID: P35227
• Protein Accession #: NP_009075.1
• Molecular Weight: 37.8