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Goat Anti-LIG1 / DNA ligase I Antibody (OAEB01260)

100 ug
$349.00
In Stock
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Gene Symbol:
LIG1
NCBI Gene Id:
3978
Protein Name:
DNA ligase 1
Swissprot Id:
P18858
Protein Accession #:
NP_000225.1
Alias Symbols:
LIG1, ligase I, DNA, ATP-dependent, MGC117397, MGC130025, DNA ligase I
Host:
Goat
Clonality:
Polyclonal
Application:
WB
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express LIG1.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express LIG1.
Predicted Species Reactivity:
Cow, Dog, Human, Mouse, Pig, Rat
Predicted Homology Based on Immunogen Sequence:
Cow: 92%; Dog: 85%; Human: 100%; Mouse: 92%; Pig: 78%; Rat: 100%
Complete computational species homology data:
LIG1 Antibody (OAEB01260)
Peptide Sequence:
C-RVREDKQPEQATTS
Product Format:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Reconstitution and Storage:
Aliquot and store at -20C. Minimize freezing and thawing.
Datasheets/Manuals:
Printable datasheet for anti-LIG1 antibody - OAEB01260
Purity:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Application Info:
Peptide ELISA: antibody detection limit dilution 1:16000.
Western Blot: Approx 125kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 102kDa according to NP_000225.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.1-0.3ug/ml.
Target Reference:
* Ferrari G, Rossi R, Arosio D, Vindigni A, Biamonti G, Montecucco A,; Cell cycle-dependent phosphorylation of human DNA ligase I at the cyclin-dependent kinase sites.; J. Biol. Chem. 2003 Sep 278 (39): 37761-7.; Pubmed 12851383

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