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Goat anti-LGR7 Antibody (OAEB00596)

  • Catalog#: OAEB00596
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Gene Symbol:
RXFP1
NCBI Gene Id:
59350
Protein Name:
Relaxin receptor 1
Swissprot Id:
Q9HBX9
Protein Accession #:
NP_067647.2
Host:
Goat
Clonality:
Polyclonal
Application:
ELISA
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express RXFP1.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express RXFP1.
Peptide Sequence:
CEMSLISQSTRLNSYS
Product Format:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Reconstitution and Storage:
Aliquot and store at -20C. Minimize freezing and thawing.
Datasheets/Manuals:
Printable datasheet for OAEB00596
Purity:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Application Info:
Peptide ELISA: antibody detection limit dilution 1:4000.
Western Blot: Preliminary experiments gave bands at approx 100kDa and 35kDa in Human Colon and Heart lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 87kDa according to NP_067647.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
Target Reference:
* Halls ML, Bond CP, Sudo S, Kumagai J, Ferraro T, Layfield S, Bathgate RA, Summers RJ.; Multiple binding sites revealed by interaction of relaxin family peptides with native and chimeric relaxin family peptide receptors 1 and 2 (LGR7 & LGR8).; J Pharmacol Exp Ther. 2005 Jan 12; [Epub ahead of print]; Pubmed 15649866

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