Goat Anti-KCC3 / SLC12A6 Antibody (OAEB02364)

Catalog No: OAEB02364 (Formerly GWB-CB3950)

Size:100UG

OAEB02364 (0.1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

OAEB02364 (2.5ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: WB, IHC, P-ELISA
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: SLC12A6
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Peptide Sequence: SRHIDVCSKTKE
• Concentration: 0.5 mg/ml
• Specificity: This antibody is expected to recognize all reported isoforms (NP_598408.1; NP_005126.1; NP_001035959.1; NP_001035961.1; NP_001035962.1); Reported variants represent identical protein: NP_001035959.1; NP_001035960.1
• Application Info: P-ELISA: Antibody detection limit dilution 1:16000
  WB: Approx 120 kDa band observed in Human Heart and Kidney lysates (calculated MW ranging 121-128 kDa according to NP_598408.1; NP_005126.1; NP_001035959.1; NP_001035960.1; NP_001035961.1; NP_001035962.1). Recommended concentration: 0.1-0.3 ug/ml.
  IHC: In paraffin embedded Human Heart shows staining of myocardial fibrils in transverse section. Recommended concentration, 2-4 ug/ml.
• Reference: * Simard CF, Bergeron MJ, Frenette-Cotton R, Carpentier GA, Pelchat ME, Caron L, Isenring P.; Homooligomeric and heterooligomeric associations between K+-Cl- cotransporter isoforms and between K+-Cl- and Na+-K+-Cl- cotransporters.; J Biol Chem. 2007 Jun 22;282(25):18083-93. Epub 2007 Apr 26.; Pubmed 17462999

Target Info

• Gene Symbol: SLC12A6
• Gene Full Name: solute carrier family 12 member 6
• Alias Symbols: ACCPN;electroneutral potassium-chloride cotransporter 3;KCC3;KCC3A;KCC3B;K-Cl cotransporter 3;potassium chloride cotransporter 3;potassium chloride cotransporter KCC3a-S3;potassium-chloride transporter-3a;potassium-chloride transporter-3b;solute carrier family 12 (potassium/chloride transporter), member 6;solute carrier family 12 (potassium/chloride transporters), member 6;solute carrier family 12 member 6.
• NCBI Gene Id: 9990
• Protein Name: Solute carrier family 12 (Potassium/chloride transporters), member 6 EMBL AAH70107.2
• Uniprot ID: Q6NSI7
• Protein Accession #: NP_001035959.1
• Molecular Weight: 128; 122; 121; 127; 126