Goat Anti-Human Kappa Antibody - AP Conjugated (OASB01856)

Catalog No: OASB01856

Size:1ML

ELISA plate was coated with purified human IgGk, IgMk, IgAk, IgGl, IgMl, and IgAl. Immunoglobulins were detected with serially diluted Goat Anti-Human Kappa, Mouse ads-AP.

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Host: Goat
• Conjugation: AP
• Additional Information: Description: Pooled antisera from goats hyperimmunized with human kappa light chains; purified by affinity chromatography on human kappa light chains covalently linked to agarose; reacts with human kappa light chains; cross-adsorbed against human lambda light chains / mouse immunoglobulins and pooled sera for minimal reactivity
• Reconstitution and Storage: Store at 2-8C
• Immunogen: Human kappa light chains
• Purification: Affinity chromatography on pooled human Igs with κ light chains covalently linkedto agarose.
• Concentration: Lot specific
• Specificity: Kappa
• Characterization: To ensure lot- to- lot consistency, each batch of product is tested by ELISA, Fluorescent ELISA, and/or flowcytometry for conformance to characteristics of a standard reference reagent.
• Warning: Reagents contain sodium azide which is very toxic if ingested or inhaled. Avoid contact with skin, eyes, orclothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copiousamounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxichydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water beforediscarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
• Dilution: Immunofluorescence UNLB, TRITC, TXRD and BIOT conjugates <= 1 ug/10⁶ cells
  ELISA: AP conjugate
  HRP conjugate
  BIOT conjugate
  1:2,000-1:4,000
  1:4,000-1:8,000
  1:5,000-1:20,000
• Application Info: Direct immunofluorescence staining, Enzyme-Linked-Immunosorbent-Assay (ELISA), Western blotting, Dot-and slot-immunoblotting, Immunohistochemistry
• Other Applications Data: Since applications vary, you should determine the optimum workingdilution of the product that is appropriate for your specific need.
• Storage: - The purified (UNLB) antibody is supplied as 1.0 mg purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C
  - Texas Red (TXRD) conjugates are supplied as 1.0 mg in 1.0 mL PBS/NaN3. Store at 2- 8 C
  - The rhodamine (TRITC) conjugate is supplied as 1.0 mg in 1.0 mL PBS/NaN3. Store at 2- 8 C
  - The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mMMgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2- 8 C or long- term at - 20 C
  - The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50%glycerol/50% PBS, pH 7.4. No preservative added. Store at 2- 8 C or long- term at - 20 C
  - The biotin (BIOT) conjugate is supplied as 1.0 mg in 2.0 mL PBS/NaN3. Store at 2- 8 C
  - Reagents are stable for the period shown on the label if stored as directed.
• Cross Absorption: Pooled human myeloma proteins with λ light chains and mouse immunoglobulins

Target Info

• Gene Symbol: IGKC
• Gene Full Name: Ig kappa chain C region
• Alias Symbols: Km, HCAK1, IGKCD
• NCBI Gene Id: 3514
• Uniprot ID: P01834