Human Collagen Type I Antibody : Biotin (OASB02203)

Catalog No: OASB02203

Size:200UG

Paraffin embedded human gastric cancer tissue was stained with Goat Anti-Type I Collagen-BIOT followed by Streptavidin-HRP, DAB, and hematoxylin.

Paraffin embedded human gastric cancer tissue was stained with Goat Anti-Type I Collagen-BIOT followed by Streptavidin-HRP, DAB, and hematoxylin.

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Mouse, Rat, Bovine, Canine, Chicken, Chinchilla, Elephant, Feline, Guinea Pig, Hamster, Porcine, Rabbit, Sheep, Tree Shrew
• Product Format: Liquid. Supplied as 0.2 mg in 0.5 mL (Conc = 0.4 mg/mL) of PBS with 0.1% sodium azide.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Goat
• Conjugation: Biotin
• Application: IHC-P, IHC-F, ICC, EM, FC, WB, IP
• Additional Information: Description: Pooled antisera from goats hyperimmunized with type I collagen; purified by affinity chromatography on type I collagen covalently linked to agarose; reacts with conformational determinants on type I collagen; cross-adsorbed against Collagen types II, III, IV, V, and VI for minimal reactivity
• Reconstitution and Storage: Store at 2-8C
• Immunogen: Type I collagen
• Purification: Affinity chromatography on human type I collagen covalently linked to agarose.
  Cross Absorption: Collagen types II, III, IV, V and VI covalently linked to agarose.
  
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: 0.4 mg/mL
• Specificity: Type I Collagen
• Source: Pooled antisera from goats hyperimmunized with human type I collagen.
• Warning: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
• Application Info: Dot-and slot-immunoblotting Immunocytochemistry: 1:20-1:40 Immunohistochemistry (frozen sections only), 1:20-1:40 ELISA 1:1,000-1:4,000 Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Target Info

• Gene Symbol: COL1A1, COL1A2
• Alias Symbols: OI1, OI2, OI3, OI4, EDSC, CAFYD, EDSARTH1
• NCBI Gene Id: 1277; 1278
• Protein Name: collagen alpha-1(I) chain
• Description of Target: This gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene.
• Uniprot ID: P02452, P08123
• Protein Accession #: NP_000079.2
• Nucleotide Accession #: NM_000088.3