Goat Anti-Fumarase / FH Antibody (OAEB02064)

Catalog No: OAEB02064 (Formerly GWB-9002A3)

Size:100UG

OAEB02064 (0.01ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing Fumarase (RC200614) and probed with OAEB02064 (mock transfection in first lane), tested by Origene.

Antibody Concentration :1.5ug/ml

Product Info

• Predicted Species Reactivity: Dog|Human|Mouse|Rat
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: WB
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: FH
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Horse: 100%; Human: 100%; Rat: 100%; Sheep: 100%; Zebrafish: 100%
• Peptide Sequence: C-HPNDHVNKSQSSND
• Concentration: 0.5 mg/ml
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Application Info: Peptide ELISA: antibody detection limit dilution 1:64000.
  Western Blot: Approx 48kDa band observed in Human Kidney, Liver and Lung lysates (calculated MW of 54.6kDa according to NP_000134.2). The observed molecular weight corresponds to earlier findings with antibodies from different sources. In transfected HEK293 transiently expressing Fumarase a band of approx. 55kDa is observed. This band is not observed in the non-transfected HEK293.Recommended concentration: 0.01-0.03ug/ml.
• Reference: * Pollard PJ, Spencer-Dene B, Shukla D, Howarth K, Nye E, El-Bahrawy M, Deheragoda M, Joannou M, McDonald S, Martin A, Igarashi P, Varsani-Brown S, Rosewell I, Poulsom R, Maxwell P, Stamp GW, Tomlinson IP.; Targeted inactivation of fh1 causes proliferative renal cyst development and activation of the hypoxia pathway.; Cancer Cell. 2007 Apr;11(4):311-9.; Pubmed 17418408

Target Info

• Gene Symbol: Fh|FH|Fh1
• Gene Full Name: fumarate hydratase|fumarate hydratase 1
• Alias Symbols: EF-3;epididymis secretory sperm binding protein;Fh;Fh1;Fh-1;FMRD;fuma;fumarase;fumarate hydratase 1;fumarate hydratase, mitochondrial;HLRCC;HsFH;LRCC;MCL;MCUL1.
• NCBI Gene Id: 14194|2271|24368
• Protein Name: Fumarate hydratase, mitochondrial
• Uniprot ID: P07954
• Protein Accession #: NP_000134.2
• Molecular Weight: 54.6