Goat Anti-FOXL2 / BPES Antibody (OAEB02240)

Catalog No: OAEB02240 (Formerly GWB-C59EF0)

Size:100UG

OAEB02240 (0.5ug/ml) staining of Human Ovary lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

OAEB02240 (2ug/ml) staining of paraffin embedded Human Ovary. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Product Info

• Predicted Species Reactivity: Bovine|Human|Mouse|Rat
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: IHC, WB
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: FOXL2
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Goat: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rabbit: 100%; Rat: 100%
• Peptide Sequence: C-DSKTGALHSRLDL
• Concentration: 0.5 mg/ml
• Application Info: Peptide ELISA: antibody detection limit dilution 1:32000.
  Western Blot: Approx 50kDa band observed in Human Ovary lysates (calculated MW of 38.8kDa according to NP_075555.1). Recommended concentration: 0.5-1.5ug/ml. An additional band of unknown identity was also consistently observed at 30kDa. This band was successfully blocked by incubation with the immunizing peptide.
  IHC: In paraffin embedded Ovary shows nuclear staining in select cells. Recommended concentration, 2-4ug/ml.
  
• Reference: * Blount AL, Schmidt K, Justice NJ, Vale WW, Fischer WH, Bilezikjian LM.; FoxL2 and Smad3 coordinately regulate follistatin gene transcription.; J Biol Chem. 2009 Mar 20;284(12):7631-45. Epub 2008 Dec 23.; Pubmed 19106105
  * Kalfa N, Fellous M, Boizet-Bonhoure B, Patte C, Duvillard P, Pienkowski C, Jaubert F, Ecochard A, Sultan C.; Aberrant expression of ovary determining gene FOXL2 in the testis and juvenile granulosa cell tumor in children.; J Urol. 2008 Oct;180(4 Suppl):1810-3.; Pubmed 18721930
  * Crisponi L, Deiana M, Loi A, Chiappe F, Uda M, Amati P, Bisceglia L, Zelante L, Nagaraja R, Porcu S, Ristaldi MS, Marzella R, Rocchi M, Nicolino M, Lienhardt-Roussie A, Nivelon A, Verloes A, Schlessinger D, Gasparini P, Bonneau D, Cao A, Pilia G.; The putative forkhead transcription factor FOXL2 is mutated in blepharophimosis/ptosis/epicanthus inversus syndrome.; Nat Genet. 2001 Feb;27(2):159-66.; Pubmed 11175783

Target Info

• Gene Symbol: Foxl2|FOXL2
• Gene Full Name: forkhead box L2
• Alias Symbols: AU045128;BPES;BPES1;forkhead box protein L2;forkhead transcription factor FOXL2;Pf;Pfrk;P-Frk;PINTO;pituitary forkhead factor;POF3;putative transcription factor foxl2.
• NCBI Gene Id: 26927|668
• Protein Name: Forkhead box protein L2
• Uniprot ID: P58012
• Protein Accession #: NP_075555.1
• Molecular Weight: 38.8