Size:100 ug
$299.00
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SKU
OAEB02496
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Goat Anti-FOXA1 / HNF3A Antibody (OAEB02496)

Catalog#: OAEB02496
Domestic: within 1 week delivery | International: 1 week
This product is Genway GWB-341C60
More Information
Predicted Species Reactivity Cow, Guinea Pig, Human, Mouse, Rat, Zebrafish
Product Format Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Clonality Polyclonal
Host Goat
Application WB, IHC, CHIP
Reconstitution and Storage Aliquot and store at -20C. Minimize freezing and thawing.
Replacement Item This antibody may replace item sc-101058 from Santa Cruz Biotechnology.
Immunogen Peptide with sequence C-GVYSRPVLNTS, from the C Terminus of the protein sequence according to NP_004487.2.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Predicted Homology Based on Immunogen Sequence Cow: 100%; Guinea Pig: 100%; Human: 100%; Mouse: 100%; Rat: 100%; Zebrafish: 100%
Complete computational species homology data FOXA1 Antibody (OAEB02496)
Peptide Sequence C-GVYSRPVLNTS
Datasheets/Manuals Printable datasheet for anti-FOXA1 antibody - OAEB02496
Application Info Peptide ELISA: antibody detection limit dilution 1:16000.
Western Blot: Approx 50kDa band observed in Human lysates of cell line HepG2 (calculated MW of 49.1kDa according to NP_004487.2). Recommended concentration: 0.2-0.6ug/ml. An additional band of unknown identity was also consistently observed at 35kDa. This band was successfully blocked by incubation with the immunising peptide.

IHC: In paraffin embedded Human Prostate shows preferential nuclear staining in the epithelial cells. Recommended concentration, 2-4ug/ml.
ChIP: This antibody has been successfully used for mouse embryonic stem cells and it has been confirmed in ChIP by anonymous customers.
Target Reference * Hua S, Kittler R, White KP.; Genomic antagonism between retinoic acid and estrogen signaling in breast cancer.; Cell. 2009 Jun 26;137(7):1259-71.; Pubmed 19563758
* Eeckhoute J, Lupien M, Meyer CA, Verzi MP, Shivdasani RA, Liu XS, Brown M.; Cell-type selective chromatin remodeling defines the active subset of FOXA1-bound enhancers.; Genome Res. 2009 Mar;19(3):372-80.; Pubmed 19129543
* Lupien M, Eeckhoute J, Meyer CA, Wang Q, Zhang Y, Li W, Carroll JS, Liu XS, Brown M.; FoxA1 translates epigenetic signatures into enhancer-driven lineage-specific transcription.; Cell. 2008 Mar 21;132(6):958-70.; Pubmed 18358809
* Eeckhoute J, Carroll JS, Geistlinger TR, Torres-Arzayus MI, Brown M.; A cell-type-specific transcriptional network required for estrogen regulation of cyclin D1 and cell cycle progression in breast cancer.; Genes Dev. 2006 Sep 15;20(18):2513-26.; Pubmed 16980581
* Carroll JS, Liu XS, Brodsky AS, Li W, Meyer CA, Szary AJ, Eeckhoute J, Shao W, Hestermann EV, Geistlinger TR, Fox EA, Silver PA, Brown M.; Chromosome-wide mapping of estrogen receptor binding reveals long-range regulation requiring the forkhead protein FoxA1.; Cell. 2005 Jul 15;122(1):33-43.; Pubmed 16009131
* Nguyen TT, Cho K, Stratton SA, Barton MC.; Transcription factor interactions and chromatin modifications associated with p53-mediated, developmental repression of the alpha-fetoprotein gene.; Mol Cell Biol. 2005 Mar;25(6):2147-57.; Pubmed 15743813
* Lin L, Miller CT, Contreras JI, Prescott MS, Dagenais SL, Wu R, Yee J, Orringer MB, Misek DE, Hanash SM, Glover TW, Beer DG.; The hepatocyte nuclear factor 3 alpha gene, HNF3alpha (FOXA1), on chromosome band 14q13 is amplified and overexpressed in esophageal and lung adenocarcinomas.; Cancer Res. 2002 Sep 15;62(18):5273-9.; Pubmed 12234996
Gene Symbol FOXA1
Alias Symbols FOXA1, HNF3A, forkhead box A1, TCF3A, MGC33105, hepatocyte nuclear factor 3, alpha
NCBI Gene Id 3169
Protein Name Hepatocyte nuclear factor 3-alpha
Swissprot Id P55317
Protein Accession # NP_004487.2
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express FOXA1.
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express FOXA1.
  1. What is the species homology for "Goat Anti-FOXA1 / HNF3A Antibody (OAEB02496)"?

    The tested species reactivity for this item is "". This antibody is predicted to have homology to "Cow, Guinea Pig, Human, Mouse, Rat, Zebrafish".

  2. How long will it take to receive "Goat Anti-FOXA1 / HNF3A Antibody (OAEB02496)"?

    This item is available "Domestic: within 1 week delivery | International: 1 week".

  3. What buffer format is "Goat Anti-FOXA1 / HNF3A Antibody (OAEB02496)" provided in?

    This item is provided in "Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.".
    Additional format options may be available. For more information please contact info@avivasysbio.com.

  4. What are other names for "Goat Anti-FOXA1 / HNF3A Antibody (OAEB02496)"?

    This target may also be called "FOXA1, HNF3A, forkhead box A1, TCF3A, MGC33105, hepatocyte nuclear factor 3, alpha" in publications.

  5. What is the shipping cost for "Goat Anti-FOXA1 / HNF3A Antibody (OAEB02496)"?

    The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.

  6. What is the guarantee for "Goat Anti-FOXA1 / HNF3A Antibody (OAEB02496)"?

    All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.

  7. Can I get bulk pricing for "Goat Anti-FOXA1 / HNF3A Antibody (OAEB02496)"?

    You can get bulk pricing for this item by going here.

  8. What is the molecular weight of the protein?

    The molecular weight reported by Uniprot for this item is "".
    Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.

  9. What protocols are available for "Goat Anti-FOXA1 / HNF3A Antibody (OAEB02496)"?

    We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data tab on the product page.

  10. What are positive controls for "FOXA1"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  11. What are negative controls for "FOXA1"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  12. What other proteins interact with "FOXA1"?

    This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.

  13. What biological processes are associated with "FOXA1"?

    This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.

  14. What cellular components are associated with "FOXA1"?

    This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.

  15. What protein functions are associated with "FOXA1"?

    This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.

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