- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Printable datasheet for Goat Anti-FHL1 / SLIM1 Antibody (OAEB02403) |
---|
Predicted Species Reactivity | Bovine|Dog|Human|Mouse|Porcine|Rat |
---|---|
Product Format | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Clonality | Polyclonal |
Host | Goat |
Application | WB, IHC |
Reconstitution and Storage | Aliquot and store at -20C. Minimize freezing and thawing. |
Immunogen | FHL1 |
Purification | Ammonium Sulfate Precipitated; Affinity Purified |
Predicted Homology Based on Immunogen Sequence | Cow: 91%; Dog: 91%; Guinea Pig: 83%; Horse: 91%; Human: 91%; Mouse: 100%; Pig: 91%; Rabbit: 91%; Rat: 100%; Sheep: 91% |
Peptide Sequence | CNKRFVFHNEQVY |
Concentration | 0.5 mg/ml |
Purity | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Application Info | Peptide ELISA: antibody detection limit dilution 1:32000. Western Blot: Approx 30kDa band observed in Human Muscle lysates (calculated MW of 31.9kDa according to NP_001440). Recommended concentration: 0.05-0.3ug/ml. Immunohistochemistry: In paraffin embedded Human Testis shows fibrous staining in the cytoplasm of developing sperm cells.. Recommended concentration, 2-2ug/ml. |
Reference | * Schessl J, Taratuto AL, Sewry C, Battini R, Chin SS, Maiti B, Dubrovsky AL, Erro MG, Espada G, Robertella M, Saccoliti M, Olmos P, Bridges LR, Standring P, Hu Y, Zou Y, Swoboda KJ, Scavina M, Goebel HH, Mitchell CA, Flanigan KM, Muntoni F, Bonnemann CG.; Clinical, histological and genetic characterization of reducing body myopathy caused by mutations in FHL1.; Brain. 2009 Feb;132(Pt 2):452-64.; Pubmed 19181672 * Gramolini AO, Kislinger T, Alikhani-Koopaei R, Fong V, Thompson NJ, Isserlin R, Sharma P, Oudit GY, Trivieri MG, Fagan A, Kannan A, Higgins DG, Huedig H, Hess G, Arab S, Seidman JG, Seidman CE, Frey B, Perry M, Backx PH, Liu PP, MacLennan DH, Emili A.; Comparative proteomics profiling of a phospholamban mutant mouse model of dilated cardiomyopathy reveals progressive intracellular stress responses.; Mol Cell Proteomics. 2008 Mar;7(3):519-33.; Pubmed 18056057 * Brown S, McGrath MJ, Ooms LM, Gurung R, Maimone MM, Mitchell CA.; Characterization of two isoforms of the skeletal muscle LIM protein 1, SLIM1. Localization of SLIM1 at focal adhesions and the isoform slimmer in the nucleus of myoblasts and cytoplasm of myotubes suggests distinct roles in the cytoskeleton and in nuclear-cytoplasmic communication.; J Biol Chem. 1999 Sep 17;274(38):27083-91.; Pubmed 10480922 |
Gene Symbol | Fhl1|FHL1 |
---|---|
Gene Full Name | four and a half LIM domains 1 |
Alias Symbols | FCMSU;FHL-1;FHL1A;FHL1B;FLH1A;four and a half LIM domains protein 1;four-and-a-half Lin11, Isl-1 and Mec-3 domains 1;Kyo;KyoT;LIM protein SLIMMER;RAM14-1;RBMX1A;RBMX1B;RBP-associated molecule 14-1;skeletal muscle LIM-protein 1;SLIM;SLIM1;SLIM-1;SLIMMER;XMPMA. |
NCBI Gene Id | 14199|2273|25177 |
Protein Name | Four and a half LIM domains protein 1 |
Uniprot ID | Q13642 |
Protein Accession # | NP_001440.1 |
Molecular Weight | 31.9; 33.6 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
-
What is the species homology for "Goat Anti-FHL1 / SLIM1 Antibody (OAEB02403)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Bovine|Dog|Human|Mouse|Porcine|Rat".
-
How long will it take to receive "Goat Anti-FHL1 / SLIM1 Antibody (OAEB02403)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
-
What buffer format is "Goat Anti-FHL1 / SLIM1 Antibody (OAEB02403)" provided in?
This item is provided in "Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "Goat Anti-FHL1 / SLIM1 Antibody (OAEB02403)"?
This target may also be called "FCMSU;FHL-1;FHL1A;FHL1B;FLH1A;four and a half LIM domains protein 1;four-and-a-half Lin11, Isl-1 and Mec-3 domains 1;Kyo;KyoT;LIM protein SLIMMER;RAM14-1;RBMX1A;RBMX1B;RBP-associated molecule 14-1;skeletal muscle LIM-protein 1;SLIM;SLIM1;SLIM-1;SLIMMER;XMPMA." in publications.
-
What is the shipping cost for "Goat Anti-FHL1 / SLIM1 Antibody (OAEB02403)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
-
What is the guarantee for "Goat Anti-FHL1 / SLIM1 Antibody (OAEB02403)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
-
Can I get bulk pricing for "Goat Anti-FHL1 / SLIM1 Antibody (OAEB02403)"?
You can get bulk pricing for this item by going here.
-
What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "31.9; 33.6".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "Goat Anti-FHL1 / SLIM1 Antibody (OAEB02403)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
-
What are positive controls for "Fhl1|FHL1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
-
What are negative controls for "Fhl1|FHL1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
-
What other proteins interact with "Fhl1|FHL1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
-
What biological processes are associated with "Fhl1|FHL1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
-
What cellular components are associated with "Fhl1|FHL1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
-
What protein functions are associated with "Fhl1|FHL1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.