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Goat Anti-Ezrin / villin 2 Antibody (OAEB02383)

100 ug
$349.00
In Stock
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Gene Symbol:
VIL2
NCBI Gene Id:
7430
Protein Name:
Ezrin
Swissprot Id:
P15311
Protein Accession #:
NP_003370.2
Replacement Item:
This antibody may replace item sc-170691 from Santa Cruz Biotechnology.
Host:
Goat
Clonality:
Polyclonal
Application:
WB, IHC
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express VIL2.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express VIL2.
Predicted Species Reactivity:
Dog, Human
Predicted Homology Based on Immunogen Sequence:
Dog; Human
Complete computational species homology data:
VIL2 Antibody (OAEB02383)
Peptide Sequence:
C-QDYEEKTKKAER
Product Format:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Reconstitution and Storage:
Aliquot and store at -20C. Minimize freezing and thawing.
Datasheets/Manuals:
Printable datasheet for anti-VIL2 antibody - OAEB02383
Purity:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Application Info:
Peptide ELISA: antibody detection limit dilution 1:16000.
Western Blot: Approx 80kDa band observed in Human Duodenum and Placenta lysates (calculated MW of 69.4kDa according to NP_003370.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Zhu et al, Am J Physiol Cell Physiol. 2005 Jun;288(6):C1242-54.; PMID: 15788482). Recommended concentration: 1-3ug/ml.
Immunohistochemistry: In paraffin embedded Human Small Intestine shows strong staining of enterocytes. Recommended concentration, 3-5ug/ml.
Target Reference:
* Stanasila L, Abuin L, Diviani D, Cotecchia S.; Ezrin directly interacts with the alpha1b-adrenergic receptor and plays a role in receptor recycling.; J Biol Chem. 2006 Feb 17;281(7):4354-63. Epub 2005 Dec 13.; Pubmed 16352594

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