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Goat Anti-ERK2 / MAPK1 Antibody (OAEB02222)

100 ug
$349.00
In Stock
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Gene Symbol:
MAPK1
NCBI Gene Id:
5594
Protein Name:
Mitogen-activated protein kinase 1
Swissprot Id:
P28482
Protein Accession #:
NP_002736.3
Alias Symbols:
MAPK1, ERK2, ERK, p38, p40, p41, ERT1, MAPK2, PRKM1, PRKM2, P42MAPK, p41mapk, mitogen-activated protein kinase 1, protein tyrosine kinase ERK2, mitogen-activated protein kinase 2, extracellular signal-regulated kinase 2, extracellular sign
Replacement Item:
This antibody may replace item sc-135900 from Santa Cruz Biotechnology.
Host:
Goat
Clonality:
Polyclonal
Application:
WB
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express MAPK1.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express MAPK1.
Immunogen:
This antibody is expected to recognise an epitope corresponding to aa 9-19 of both reported protein isoforms of human ERK2 / MAPK1 protein.
Predicted Species Reactivity:
Dog, Human, Mouse, Pig, Rat
Predicted Homology Based on Immunogen Sequence:
Dog: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rat: 100%
Complete computational species homology data:
MAPK1 Antibody (OAEB02222)
Peptide Sequence:
CAAGPEMVRGQVF
Product Format:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Reconstitution and Storage:
Aliquot and store at -20C. Minimize freezing and thawing.
Datasheets/Manuals:
Printable datasheet for anti-MAPK1 antibody - OAEB02222
Purity:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Application Info:
Peptide ELISA: antibody detection limit dilution 1:8000.
Western Blot: Approx 38kDa band observed in human cervix epitheloid carcinoma HeLa and and human hepatoblastoma HepG2 lysates (calculated MW of 41.4kDa according to NP_002736 and NP_620407). Recommended concentration: 0.5-2ug/ml.
Target Reference:
* Lee SM, Nguyen TH, Park MH, Kim KS, Cho KJ, Moon DC, Kim HY, Yoon do Y, Hong JT.; EPO receptor-mediated ERK kinase and NF-kappaB activation in erythropoietin-promoted differentiation of astrocytes.; Biochem Biophys Res Commun. 2004 Aug 6;320(4):1087-95.; Pubmed 15249201

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