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Goat Anti-ENPP1 / PC1 Antibody (OAEB02445)

100 ug
$349.00
In Stock
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Gene Symbol:
ENPP1
NCBI Gene Id:
5167
Protein Name:
Ectonucleotide pyrophosphatase/phosphodiesterase family member 1
Swissprot Id:
P22413
Protein Accession #:
NP_006199.2
Alias Symbols:
ENPP1, PC-1, ectonucleotide pyrophosphatase/phosphodiesterase 1, HGNC:3356, M6S1, NPP1, NPPS, PCA1, PDNP1, Ly-41 antigen, OTTHUMP00000043194, alkaline phosphodiesterase 1, membrane component, chromosome 6, surface marker 1, phosphodiesterase
Replacement Item:
This antibody may replace item sc-166649 from Santa Cruz Biotechnology.
Host:
Goat
Clonality:
Polyclonal
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Application:
WB, IHC
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express ENPP1.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express ENPP1.
Immunogen:
Peptide with sequence C-KTHLPTFSQED, from the C Terminus of the protein sequence according to NP_006199.2.
Predicted Species Reactivity:
Cow, Human, Mouse, Rabbit, Rat
Predicted Homology Based on Immunogen Sequence:
Cow: 90%; Human: 100%; Mouse: 90%; Rabbit: 100%; Rat: 90%
Complete computational species homology data:
ENPP1 Antibody (OAEB02445)
Peptide Sequence:
C-KTHLPTFSQED
Product Format:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Reconstitution and Storage:
Aliquot and store at -20C. Minimize freezing and thawing.
Datasheets/Manuals:
Printable datasheet for anti-ENPP1 antibody - OAEB02445
Application Info:
Peptide ELISA: antibody detection limit dilution 1:32000.
Western Blot: In transfected HEK293 transiently expressing ENPP1 a band of approx. 100 kDa is observed. This band is not observed in the non-transfected HEK293. The calculated molecular size is 105kDa according to NP_006199.2. Recommended concentration, 1-3ug/ml.
Immunohistochemistry: In paraffin embedded Liver shows membranous staining in many hepatocytes. This antibody has also been successfully used on Human Pancreas showing membranous staining of acinar cells. Recommended concentration, 1-2ug/ml.
Additional Information:
Reactivity Info: Tested: Human; Expected: Human, Mouse, Rat
Target Reference:
* Orriss IR, Utting JC, Brandao-Burch A, Colston K, Grubb BR, Burnstock G, Arnett TR.; Extracellular nucleotides block bone mineralization in vitro: evidence for dual inhibitory mechanisms involving both P2Y2 receptors and pyrophosphate.; Endocrinology. 2007 Sep;148(9):4208-16.; Pubmed 17569759
* Meyre D, Bouatia-Naji N, Tounian A, Samson C, Lecoeur C, Vatin V, Ghoussaini M, Wachter C, Hercberg S, Charpentier G, Patsch W, Pattou F, Charles MA, Tounian P, Clement K, Jouret B, Weill J, Maddux BA, Goldfine ID, Walley A, Boutin P, Dina C, Froguel P.; Variants of ENPP1 are associated with childhood and adult obesity and increase the risk of glucose intolerance and type 2 diabetes.; Nat Genet. 2005 Aug;37(8):863-7. Epub 2005 Jul 17.; Pubmed 16025115
Publications:

de la Croix Ndong, J. et al. Asfotase-alpha improves bone growth, mineralization and strength in mouse models of neurofibromatosis type-1. Nat. Med. 20, 904-10 (2014). WB, IHC, Cow, Human, Mouse, Rabbit, Rat 24997609

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