Size:100 ug
$299.00
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OAEB02445
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Goat Anti-ENPP1 / PC1 Antibody (OAEB02445)

Catalog#: OAEB02445
Domestic: within 1 week delivery International: 1 week
This product is Genway GWB-94E204
More Information
Tested Species Reactivity Human
Predicted Species Reactivity Human, Mouse, Rat
Product Format Liquid Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Clonality Polyclonal
Host Goat
Application P-IHC, WB
Reconstitution and Storage Aliquot and store at -20°C. Minimize freezing and thawing.
Replacement Item This antibody may replace item sc-166649 from Santa Cruz Biotechnology.
Immunogen Peptide with sequence C-KTHLPTFSQED, from the C Terminus of the protein sequence according to NP_006199.2.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Complete computational species homology data ENPP1 Antibody (OAEB02445)
Concentration 0.5 mg/ml
Datasheets/Manuals Printable datasheet for anti-ENPP1 antibody - OAEB02445
Application Info P-ELISA: Antibody detection limit dilution 1:128000
IHC: In paraffin embedded Human Liver shows membranous staining in many hepatocytes. Recommended concentration, 2-4 ug/ml.
Application Data This antibody has been successfully used in Western blot and IHC on Human:
Lau WM, Doucet M, Stadel R, Huang D, Weber KL, Kominsky SL
Enpp1: a potential facilitator of breast cancer bone metastasis.
PLoS One. 2013 Jul 5;8(7):e66752.
PMID: 23861746
This antibody has been successfully used in Western blot on Human:
Simão AM, Yadav MC, Narisawa S, Bolean M, Pizauro JM, Hoylaerts MF, Ciancaglini P, Millán JL.
Proteoliposomes harboring alkaline phosphatase and nucleotide pyrophosphatase as matrix vesicle biomimetics.
J Biol Chem. 2010 Mar 5;285(10):7598-609.
PMID: 20048161.
Target Reference * Orriss IR, Utting JC, Brandao-Burch A, Colston K, Grubb BR, Burnstock G, Arnett TR.; Extracellular nucleotides block bone mineralization in vitro: evidence for dual inhibitory mechanisms involving both P2Y2 receptors and pyrophosphate.; Endocrinology. 2007 Sep;148(9):4208-16.; Pubmed 17569759
* Meyre D, Bouatia-Naji N, Tounian A, Samson C, Lecoeur C, Vatin V, Ghoussaini M, Wachter C, Hercberg S, Charpentier G, Patsch W, Pattou F, Charles MA, Tounian P, Clement K, Jouret B, Weill J, Maddux BA, Goldfine ID, Walley A, Boutin P, Dina C, Froguel P.; Variants of ENPP1 are associated with childhood and adult obesity and increase the risk of glucose intolerance and type 2 diabetes.; Nat Genet. 2005 Aug;37(8):863-7. Epub 2005 Jul 17.; Pubmed 16025115
Publications

de la Croix Ndong, J. et al. Asfotase-alpha improves bone growth, mineralization and strength in mouse models of neurofibromatosis type-1. Nat. Med. 20, 904-10 (2014). WB, IHC, Cow, Human, Mouse, Rabbit, Rat 24997609

Gene Symbol ENPP1
Alias Symbols ENPP1, PC-1, ectonucleotide pyrophosphatase/phosphodiesterase 1, HGNC:3356, M6S1, NPP1, NPPS, PCA1, PDNP1, Ly-41 antigen, OTTHUMP00000043194, alkaline phosphodiesterase 1, membrane component, chromosome 6, surface marker 1, phosphodiesterase
NCBI Gene Id 5167
Protein Name Ectonucleotide pyrophosphatase/phosphodiesterase family member 1
Swissprot Id P22413
Protein Accession # NP_006199.2
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express ENPP1.
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express ENPP1.
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