Goat Anti-ENPP1 / PC1 Antibody (OAEB02445)

Catalog No: OAEB02445 (Formerly GWB-94E204)

Size:100ug

HEK293 overexpressing Human ENPP1 (RC209222) and probed with OAEB02445 (mock transfection in first lane), tested by Origene.

OAEB02445 (1ug/ml) staining of paraffin embedded Human Liver. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.

(8ug/ml) staining of paraffin embedded Human Thyroid Gland. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Negative Control showing staining of paraffin embedded Human Thyroid Gland, with no primary antibody.

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Clonality: Polyclonal
• Host: Goat
• Application: IHC, P-ELISA, WB
• Reconstitution and Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Immunogen: Peptide with sequence C-KTHLPTFSQED, from the C Terminus of the protein sequence according to NP_006199.2.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Concentration: 0.5 mg/ml
• Application Info: P-ELISA: Antibody detection limit dilution 1:32000
  IHC: Paraffin embedded Human Thyroid Gland. Recommended concentration: 8 ug/mL.
• Application Data: This antibody has been successfully used in Western blot and IHC:
  Lau WM, Doucet M, Stadel R, Huang D, Weber KL, Kominsky SL
  Enpp1: a potential facilitator of breast cancer bone metastasis.
  PLoS One. 2013 Jul 5;8(7):e66752.
  PMID: 23861746
  This antibody has been successfully used in Western blot:
  Simão AM, Yadav MC, Narisawa S, Bolean M, Pizauro JM, Hoylaerts MF, Ciancaglini P, Millán JL.
  Proteoliposomes harboring alkaline phosphatase and nucleotide pyrophosphatase as matrix vesicle biomimetics.
  J Biol Chem. 2010 Mar 5;285(10):7598-609.
  PMID: 20048161.
• Reference: * Orriss IR, Utting JC, Brandao-Burch A, Colston K, Grubb BR, Burnstock G, Arnett TR.; Extracellular nucleotides block bone mineralization in vitro: evidence for dual inhibitory mechanisms involving both P2Y2 receptors and pyrophosphate.; Endocrinology. 2007 Sep;148(9):4208-16.; Pubmed 17569759
  * Meyre D, Bouatia-Naji N, Tounian A, Samson C, Lecoeur C, Vatin V, Ghoussaini M, Wachter C, Hercberg S, Charpentier G, Patsch W, Pattou F, Charles MA, Tounian P, Clement K, Jouret B, Weill J, Maddux BA, Goldfine ID, Walley A, Boutin P, Dina C, Froguel P.; Variants of ENPP1 are associated with childhood and adult obesity and increase the risk of glucose intolerance and type 2 diabetes.; Nat Genet. 2005 Aug;37(8):863-7. Epub 2005 Jul 17.; Pubmed 16025115
• Publications:

  de la Croix Ndong, J. et al. Asfotase-a improves bone growth, mineralization and strength in mouse models of neurofibromatosis type-1. Nat. Med. 20, 904-10 (2014). 24997609

Target Info

• Gene Symbol: ENPP1
• Alias Symbols: M6S1, NPP1, NPPS, PC-1, PCA1, ARHR2, COLED, PDNP1
• NCBI Gene Id: 5167
• Protein Name: Ectonucleotide pyrophosphatase/phosphodiesterase family member 1
• Uniprot ID: P22413
• Protein Accession #: NP_006199.2