Goat Anti-COP1 / PSEUDO-ICE (Isofom 2) Antibody (OAEB00579)

Catalog No: OAEB00579 (Formerly GWB-564B0D)

Size:100UG

Product Info

• Predicted Species Reactivity: Human
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: P-WB
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: CARD16
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human
• Peptide Sequence: C-ETLGLSAGPIPGN
• Concentration: 0.5 mg/ml
• Specificity: This antibody is expected to recognize isoform 2 (NP_443121.1) only. In addition there may be cross-reaction with INCA (GeneID 440068; NP_001007233.1).
• Application Info: P-ELISA: Antibody detection limit dilution 1:4000
  WB: Preliminary experiments gave an approx. 28-30 kDa band in human colon, pancreas, placenta and tonsil lysates after 0.3 ug/ml antibody staining. Currently, an explanation cannot be found in the literature for the band observed, given the calculated size of 10.7 kDa according to NP_443121.1. The 28-30kDa band was successfully blocked by incubation with the immunizing peptide.
• Reference: * Druilhe A, Srinivasula SM, Razmara M, Ahmad M, Alnemri ES.; Regulation of IL-1beta generation by Pseudo-ICE and ICEBERG, two dominant negative caspase recruitment domain proteins.; Cell Death Differ. 2001 Jun;8(6):649-57.; Pubmed 11536016

Target Info

• Gene Symbol: CARD16
• Gene Full Name: caspase recruitment domain family member 16
• Alias Symbols: CARD only domain-containing protein 1;CARD only protein;CARD-only protein 1;caspase recruitment domain-containing protein 16;caspase recruitment domain-only protein 1;caspase-1 dominant-negative inhibitor pseudo-ICE;caspase-1 inhibitor COP;COP;COP1;LLID-114769;pseudo interleukin-1 beta converting enzyme;pseudo interleukin-1beta converting enzyme;PSEUDO-ICE;pseudo-IL1B-converting enzyme.
• NCBI Gene Id: 114769
• Protein Name: Caspase recruitment domain-containing protein 16
• Uniprot ID: Q5EG05
• Protein Accession #: NP_443121.1
• Molecular Weight: 10.7