Goat Anti-CGI58 / ABHD5 Antibody (OAEB01343)

Catalog No: OAEB01343 (Formerly GWB-30799C)

Size:100UG

OAEB01343 (0.2ug/ml) staining of NIH3T3 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Info

• Predicted Species Reactivity: Dog|Human|Mouse|Rat
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: WB, P-IHC
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: ABHD5
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human, Mouse, Rat, Dog
• Peptide Sequence: C-FPERPDLADQDR
• Concentration: 0.5 mg/ml
• Specificity: This antibody is not expected to cross-react with ABHD4.
• Application Info: P-ELISA: Antibody detection limit dilution 1:16000
  WB: Approx 40kDa band observed in lysates of cell lines NIH3T3 and A431 (calculated MW of 39.1kDa according to NP_057090.2). Recommended concentration: 0.2-0.6 ug/ml. Additional faint bands of 26kDa and 18kDa were observed. These bands were successfully blocked by incubation with the immunizing peptide.
  IHC: Paraffin embedded Human Pancreas and Kidney. Recommended concentration: 3.75 ug/ml.
• Reference: * Lass A, Zimmermann R, Haemmerle G, Riederer M, Schoiswohl G, Schweiger M, Kienesberger P, Strauss JG, Gorkiewicz G, Zechner R.; Adipose triglyceride lipase-mediated lipolysis of cellular fat stores is activated by CGI-58 and defective in Chanarin-Dorfman Syndrome.; Cell Metab. 2006 May;3(5):309-19.; Pubmed 16679289
• Publications:

  Skinner, J. R., Harris, L.-A. L. S., Shew, T. M., Abumrad, N. A. & Wolins, N. E. Perilipin 1 moves between the fat droplet and the endoplasmic reticulum. Adipocyte 2, 80-6 (2013). 23805403

Target Info

• Gene Symbol: Abhd5|ABHD5
• Gene Full Name: abhydrolase domain containing 5|abhydrolase domain containing 5, lysophosphatidic acid acyltransferase
• Alias Symbols: 1300003D03Rik;1-acylglycerol-3-phosphate O-acyltransferase ABHD5;2010002J10Rik;abhydrolase domain-containing protein 5;CGI-5;CGI58;CGI-58;IEC;IECN2;IECN5;lipid droplet-binding protein CGI-58;NCI;NCIE2;truncated abhydrolase domain-containing protein 5.
• NCBI Gene Id: 316122|51099|67469
• Protein Name: 1-acylglycerol-3-phosphate O-acyltransferase ABHD5
• Uniprot ID: Q8WTS1
• Protein Accession #: NP_057090.2
• Molecular Weight: 39.1