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Goat Anti-CGI58 / ABHD5 Antibody (OAEB01343)

Catalog#: OAEB01343
Domestic: within 1 week delivery | International: 1 week
This product is Genway GWB-30799C
More Information
Predicted Species ReactivityHuman, Mouse
Product FormatLiquid Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
ApplicationWB, P-IHC
Reconstitution and StorageAliquot and store at -20C. Minimize freezing and thawing.
Replacement ItemThis antibody may replace item sc-100468 from Santa Cruz Biotechnology.
ImmunogenPeptide with sequence C-FPERPDLADQDR, from the internal region of the protein sequence according to NP_057090.2.
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Predicted Homology Based on Immunogen SequenceHuman, Mouse, Rat, Dog
Complete computational species homology dataABHD5 Antibody (OAEB01343)
Concentration0.5 mg/ml
Datasheets/ManualsPrintable datasheet for anti-ABHD5 antibody - OAEB01343
SpecificityThis antibody is not expected to cross-react with ABHD4.
Application InfoP-ELISA: Antibody detection limit dilution 1:16000
WB: Approx 40kDa band observed in lysates of cell lines NIH3T3 and A431 (calculated MW of 39.1kDa according to NP_057090.2). Recommended concentration: 0.2-0.6 ug/ml. Additional faint bands of 26kDa and 18kDa were observed. These bands were successfully blocked by incubation with the immunizing peptide.
IHC: Paraffin embedded Human Pancreas and Kidney. Recommended concentration: 3.75 ug/ml.
Target Reference* Lass A, Zimmermann R, Haemmerle G, Riederer M, Schoiswohl G, Schweiger M, Kienesberger P, Strauss JG, Gorkiewicz G, Zechner R.; Adipose triglyceride lipase-mediated lipolysis of cellular fat stores is activated by CGI-58 and defective in Chanarin-Dorfman Syndrome.; Cell Metab. 2006 May;3(5):309-19.; Pubmed 16679289

Skinner, J. R., Harris, L.-A. L. S., Shew, T. M., Abumrad, N. A. & Wolins, N. E. Perilipin 1 moves between the fat droplet and the endoplasmic reticulum. Adipocyte 2, 80-6 (2013). WB, P-ELISA, IHC, Human, Mouse 23805403

Gene SymbolABHD5
Alias SymbolsABHD5, CGI58 protein, aCGI58, bhydrolase domain containing 5, CDS, IECN2, MGC8731, NCIE2
NCBI Gene Id51099
Protein Name1-acylglycerol-3-phosphate O-acyltransferase ABHD5
Swissprot IdQ8WTS1
Protein Accession #NP_057090.2
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express ABHD5.
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express ABHD5.
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