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Goat Anti-CGI58 / ABHD5 Antibody (OAEB01343)

100 ug
$289.00
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Gene Symbol:
ABHD5
NCBI Gene Id:
51099
Protein Name:
1-acylglycerol-3-phosphate O-acyltransferase ABHD5
Swissprot Id:
Q8WTS1
Protein Accession #:
NP_057090.2
Alias Symbols:
ABHD5, CGI58 protein, aCGI58, bhydrolase domain containing 5, CDS, IECN2, MGC8731, NCIE2
Replacement Item:
This antibody may replace item sc-100468 from Santa Cruz Biotechnology.
Host:
Goat
Clonality:
Polyclonal
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Application:
WB, P-ELISA, IHC
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express ABHD5.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express ABHD5.
Immunogen:
Peptide with sequence C-FPERPDLADQDR, from the internal region of the protein sequence according to NP_057090.2.
Predicted Homology Based on Immunogen Sequence:
Human, Mouse, Rat, Dog
Complete computational species homology data:
ABHD5 Antibody (OAEB01343)
Product Format:
Liquid Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Reconstitution and Storage:
Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
0.5 mg/ml
Datasheets/Manuals:
Printable datasheet for anti-ABHD5 antibody - OAEB01343
Specificity:
This antibody is not expected to cross-react with ABHD4.
Application Info:
P-ELISA: Antibody detection limit dilution 1:16000
WB: Approx 40kDa band observed in lysates of cell lines NIH3T3 and A431 (calculated MW of 39.1kDa according to NP_057090.2). Recommended concentration: 0.2-0.6 ug/ml. Additional faint bands of 26kDa and 18kDa were observed. These bands were successfully blocked by incubation with the immunizing peptide.
IHC: Paraffin embedded Human Pancreas and Kidney. Recommended concentration: 3.75 ug/ml.
Target Reference:
* Lass A, Zimmermann R, Haemmerle G, Riederer M, Schoiswohl G, Schweiger M, Kienesberger P, Strauss JG, Gorkiewicz G, Zechner R.; Adipose triglyceride lipase-mediated lipolysis of cellular fat stores is activated by CGI-58 and defective in Chanarin-Dorfman Syndrome.; Cell Metab. 2006 May;3(5):309-19.; Pubmed 16679289
Publications:

Skinner, J. R., Harris, L.-A. L. S., Shew, T. M., Abumrad, N. A. & Wolins, N. E. Perilipin 1 moves between the fat droplet and the endoplasmic reticulum. Adipocyte 2, 80-6 (2013). WB, P-ELISA, IHC, Human, Mouse 23805403

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