Goat Anti-CBX5 / HP1-Alpha Antibody (OAEB02359)

Catalog No: OAEB02359 (Formerly GWB-9B722D)

Size:100UG

(0.03ug/ml) staining of A43N (A) and K562 (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

OAEB02359 (0.03ug/ml) staining of Hela lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

OAEB02359 (2.5ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue)

Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml). IgG control: Unimmunized goat IgG (black line) fol

Product Info

• Predicted Species Reactivity: Dog|Human|Mouse
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: WB, IHC
• Additional Information: Reactivity Info: Tested: Human; Expected: Human, Mouse, Dog
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: CBX5
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 93%; Human: 100%; Mouse: 93%; Rat: 93%; Sheep: 100%
• Peptide Sequence: C-NKRKSNFSNSADDIK
• Concentration: 0.5 mg/ml
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Application Info: Peptide ELISA: antibody detection limit dilution 1:128000.
  Western Blot: Approx. 22kDa band observed in lysates of Human fibroblast cell lines A431 and HeLa (calculated MW of 22.2kDa according to NP_036249.1). Recommended concentration: 0.03-0.1ug/ml.
  Immunohistochemistry: In paraffin embedded Human Uterus shows strong nuclear staining in the glandular epithelial cells. Recommended concentration, 2-3ug/ml.
• Reference: * Rohr O, Lecestre D, Chasserot-Golaz S, Marban C, Avram D, Aunis D, Leid M, Schaeffer E.; Recruitment of Tat to heterochromatin protein HP1 via interaction with CTIP2 inhibits human immunodeficiency virus type 1 replication in microglial cells.; J Virol. 2003 May;77(9):5415-27.; Pubmed 12692243

Target Info

• Gene Symbol: CBX5
• Gene Full Name: chromobox 5
• Alias Symbols: antigen p25;chromobox homolog 5 (HP1 alpha homolog, Drosophila);chromobox protein homolog 5;epididymis luminal protein 25;HEL25;heterochromatin protein 1 homolog alpha;heterochromatin protein 1-alpha;HP1;HP1 alpha homolog;HP1A;HP1-ALPHA;HP1Hs alpha.
• NCBI Gene Id: 23468
• Protein Name: Chromobox protein homolog 5
• Uniprot ID: P45973
• Protein Accession #: NP_036249.1
• Molecular Weight: 22.2