Goat Anti-Catalase / CAT Antibody (OAEB02426)

Catalog No: OAEB02426 (Formerly GWB-49DC73)

Size:100ug

OAEB02426 (1ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

OAEB02426 (2ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Clonality: Polyclonal
• Host: Goat
• Application: WB
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: Peptide with sequence C-DKYNAEKPKNAIHT, from the internal region of the protein sequence according to NP_001743.1.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Predicted Homology Based on Immunogen Sequence: Human, Mouse, Dog
• Concentration: 0.5 mg/ml
• Application Info: P-ELISA: Antibody detection limit dilution 1:8000
  WB: Approx 60 kDa band observed in Human Kidney lysates (calculated MW of 59.8 kDa according to NP_001743.1). Recommended concentration: 0.3-1 ug/ml. Primary incubation was 1 hour.
• Reference: * Schriner SE, Linford NJ, Martin GM, Treuting P, Ogburn CE, Emond M, Coskun PE, Ladiges W, Wolf N, Van Remmen H, Wallace DC, Rabinovitch PS.; Extension of murine life span by overexpression of catalase targeted to mitochondria.; Science. 2005 Jun 24;308(5730):1909-11. Epub 2005 May 5.; Pubmed 15879174
• Publications:

  Imatinib Is Protective Against Ischemia-Reperfusion Injury in an Ex Vivo Rabbit Model of Lung Injury. Ann Thorac Surg. 105, 950-956 (2018). 29289364

Target Info

• Gene Symbol: CAT
• Alias Symbols: CAT, catalase, HGNC:1516, MGC138422, MGC138424
• NCBI Gene Id: 847
• Protein Name: Catalase
• Uniprot ID: P04040
• Protein Accession #: NP_001743.1