Goat Anti-C14orf169 / NO66 Antibody (OAEB02105)

Catalog No: OAEB02105 (Formerly GWB-EV0057)

Size:100UG

(0.3ug/ml) staining of A431 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

OAEB02105 (0.3ug/ml) staining of A431 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

OAEB02105 (0.3ug/ml) staining of A431 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: P-ELISA, WB
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: RIOX1
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Dog: 100%
• Peptide Sequence: C-GAQHSDSKDPR
• Concentration: 0.5 mg/ml
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Application Info: Peptide ELISA: antibody detection limit dilution 1:64000.
  Western Blot: Approx 70kDa band observed in lysates of cell line A431 (calculated MW of 71.1kDa according to NP_078920.2). Recommended concentration: 0.3-1ug/ml.
  An additional band of unknown identity was also consistently observed at 45kDa. This band was successfully blocked by incubation with the immunising peptide.
• Reference: * Eilbracht J, Reichenzeller M, Hergt M, Schnolzer M, Heid H, Stohr M, Franke WW, Schmidt-Zachmann MS.; NO66, a highly conserved dual location protein in the nucleolus and in a special type of synchronously replicating chromatin.; Mol Biol Cell. 2004 Apr;15(4):1816-32. Epub 2004 Jan 23.; Pubmed 14742713

Target Info

• Gene Symbol: Riox1|RIOX1
• Gene Full Name: ribosomal oxygenase 1
• Alias Symbols: 2410016O06Rik;60S ribosomal protein L8 histidine hydroxylase;bifunctional lysine-specific demethylase and histidyl-hydroxylase NO66;C14orf169;histone lysine demethylase NO66;hsNO66;JMJD9;lysine-specific demethylase NO66;MAPJD;myc associated protein with JmjC domain;MYC-associated protein with JmjC domain;NO;NO66;nucleolar protein 66;ribosomal oxygenase 1;ribosomal oxygenase NO66;ROX;up-regulated in lung cancer 2;URLC2.
• NCBI Gene Id: 71952|79697
• Protein Name: Lysine-specific demethylase NO66
• Uniprot ID: Q9H6W3
• Protein Accession #: NP_078920.2
• Molecular Weight: 71.1