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Datasheets/Manuals | Printable datasheet for Goat Anti-BOULE / BOLL Antibody (OAEB00409) |
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Predicted Species Reactivity | Bovine|Dog|Human |
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Product Format | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Clonality | Polyclonal |
Host | Goat |
Application | IHC |
Reconstitution and Storage | Aliquot and store at -20C. Minimize freezing and thawing. |
Immunogen | BOLL |
Purification | Ammonium Sulfate Precipitated; Affinity Purified |
Predicted Homology Based on Immunogen Sequence | Cow: 100%; Dog: 100%; Human: 100%; Mouse: 100% |
Peptide Sequence | C-QPEPIKTVWSIHY |
Concentration | 0.5 mg/ml |
Purity | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Application Info | Peptide ELISA: antibody detection limit dilution 1:32000. Western Blot: Preliminary experiments gave an approx 22kDa band in Human Testis lysates after 1.5 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 32.6kDa according to NP_932074.1 and 31.3kDa according to NP_149019.1. The 22kDa band was successfully blocked by incubation with the immunizing peptide. However, literature reports a 36kDa band in Human and Mouse Testes (PMID: 11390979). Have any further splice variants/modified forms been reported? |
Reference | * Xu EY, Moore FL, Pera RA.; A gene family required for human germ cell development evolved from an ancient meiotic gene conserved in metazoans.; Proc Natl Acad Sci U S A. 2001 Jun 19;98(13):7414-9.; Pubmed 11390979 |
Gene Symbol | BOLL |
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Gene Full Name | boule homolog, RNA binding protein |
Alias Symbols | bol, boule-like;BOULE;boule-like RNA binding protein;protein boule-like. |
NCBI Gene Id | 66037 |
Protein Name | Protein boule-like |
Uniprot ID | Q8N9W6 |
Protein Accession # | NP_932074.1 |
Molecular Weight | 32.6; 31.3 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "Goat Anti-BOULE / BOLL Antibody (OAEB00409)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Bovine|Dog|Human".
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How long will it take to receive "Goat Anti-BOULE / BOLL Antibody (OAEB00409)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "Goat Anti-BOULE / BOLL Antibody (OAEB00409)" provided in?
This item is provided in "Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "Goat Anti-BOULE / BOLL Antibody (OAEB00409)"?
This target may also be called "bol, boule-like;BOULE;boule-like RNA binding protein;protein boule-like." in publications.
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What is the shipping cost for "Goat Anti-BOULE / BOLL Antibody (OAEB00409)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "Goat Anti-BOULE / BOLL Antibody (OAEB00409)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "Goat Anti-BOULE / BOLL Antibody (OAEB00409)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "32.6; 31.3".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "Goat Anti-BOULE / BOLL Antibody (OAEB00409)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "BOLL"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "BOLL"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "BOLL"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "BOLL"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "BOLL"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "BOLL"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.