Goat Anti-BAF57 / SMARCE1 Antibody (OAEB02380)

Catalog No: OAEB02380 (Formerly GWB-2A0C65)

Size:100UG

In paraffin embedded Human Prostate shows nuclear staining in secretory epithelial cells. Recommended concentration, 3-5ug/ml.

Product Info

• Predicted Species Reactivity: Human|Mouse
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: WB, IHC
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: SMARCE1
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Dog: 92%; Guinea Pig: 83%; Horse: 100%; Human: 100%; Mouse: 92%; Rat: 92%
• Peptide Sequence: C-PPTDPIPEDEKKE
• Concentration: 0.5 mg/ml
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Application Info: Peptide ELISA: antibody detection limit dilution 1:16000.
  Western Blot: Approx 50kDa band observed in lysates of cell lines HEPG2, HeLa and Jurkat (calculated MW of 46.6kDa according to NP_003070.3). A 48kDa band was observed in transfected HEK293 transiently expressing BAF57. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.2-0.6 ug/ml.
  Full caption:
  A) HEK293 overexpressing BAF57 (RC209444) and probed (mock transfection in first lane), tested by Origene.
  B) staining (0.2ug/ml) of Jurkat lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
• Reference: * Wang W, Chi T, Xue Y, Zhou S, Kuo A, Crabtree GR.; Architectural DNA binding by a high-mobility-group/kinesin-like subunit in mammalian SWI/SNF-related complexes.; Proc Natl Acad Sci U S A. 1998 Jan 20;95(2):492-8.; Pubmed 9435219

Target Info

• Gene Symbol: Smarce1|SMARCE1
• Gene Full Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1
• Alias Symbols: 2810417B20Rik;5830412H02Rik;9030408N19Rik;Baf27;BAF5;BAF57;BRG1-associated factor 57;chromatin remodeling complex BRG1-associated factor 57;CSS5;SWI/SNF-related matrix-associated actin-dependent regulator chromatin subfamily E member 1;SWI/SNF-related matrix-associated actin-dependent regulator of chromatin e1;SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1.
• NCBI Gene Id: 57376|6605
• Protein Name: SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1
• Uniprot ID: Q969G3
• Protein Accession #: NP_003070.3
• Molecular Weight: 46.6