Goat Anti-ATP6IP2 / Renin receptor Antibody (OAEB01325)

Catalog No: OAEB01325 (Formerly GWB-60ECDB)

Size:100ug

OAEB01325 staining (0.5ug/ml) of Human Kidney lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Clonality: Polyclonal
• Host: Goat
• Application: WB, IHC
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: Peptide with sequence C-SIIYRMTNQKIRMD, from the C Terminus of the protein sequence according to NP_005756.2.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Predicted Homology Based on Immunogen Sequence: Human, Rat
• Concentration: 0.5 mg/ml
• Application Info: P-ELISA: Antibody detection limit dilution 1:16000
  WB: Approx 37 kDa band observed in Human Kidney lysates (calculated MW of 39.0kDa according to NP_005756.2). Recommended concentration: 0.25-1 ug/ml
  IHC: This antibody has been successfully used on Human tissue in the following papers: Pringle et al, J Renin Angiotensin Aldosterone Syst. 2010 Aug 11, PMID: 20702505. Delforce et al, Endocr Connect. 2017 Jan;6(1):9-19, PMID: 27956412.
• Reference: * Advani A, Kelly DJ, Cox AJ, White KE, Advani SL, Thai K, Connelly KA, Yuen D, Trogadis J, Herzenberg AM, Kuliszewski MA, Leong-Poi H, Gilbert RE.; The (Pro)renin receptor: site-specific and functional linkage to the vacuolar H+-ATPase in the kidney.; Hypertension. 2009 Aug;54(2):261-9.; Pubmed 19546380
  * Schefe JH, Neumann C, Goebel M, Danser J, Kirsch S, Gust R, Kintscher U, Unger T, Funke-Kaiser H.; Prorenin engages the (pro)renin receptor like renin and both ligand activities are unopposed by aliskiren.; J Hypertens. 2008 Sep;26(9):1787-94.; Pubmed 18698213
  * Kaneshiro Y, Ichihara A, Sakoda M, Takemitsu T, Nabi AH, Uddin MN, Nakagawa T, Nishiyama A, Suzuki F, Inagami T, Itoh H.; Slowly progressive, angiotensin II-independent glomerulosclerosis in human (pro)renin receptor-transgenic rats.; J Am Soc Nephrol. 2007 Jun;18(6):1789-95.; Pubmed 17494887
  * Ludwig J, Kerscher S, Brandt U, Pfeiffer K, Getlawi F, Apps DK, Schagger H.; Identification and characterization of a novel 9.2-kDa membrane sector-associated protein of vacuolar proton-ATPase from chromaffin granules.; J Biol Chem. 1998 May 1;273(18):10939-47.; Pubmed 9556572

Target Info

• Gene Symbol: ATP6AP2
• Alias Symbols: PRR, M8-9, MRXE, RENR, XMRE, XPDS, CDG2R, HT028, MRXSH, ELDF10, ATP6IP2, MSTP009, APT6M8-9, ATP6M8-9
• NCBI Gene Id: 10159
• Protein Name: Renin receptor
• Uniprot ID: O75787
• Protein Accession #: NP_005756.2