Goat Anti-ABCC4 / MRP4 Antibody (OAEB02455)

Catalog No: OAEB02455 (Formerly GWB-49EE5F)

Size:100ug

OAEB02455 (4ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Product Info

• Predicted Species Reactivity: Human, Rat
• Product Format: Liquid. Tris saline, pH7.3 with 0.02% sodium azide and 0.5% bovine serum albumin.
• Clonality: Polyclonal
• Host: Goat
• Application: IHC-P, P-WB, IF
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: Peptide with sequence CNGQPSTLTIFETAL, from the C Terminus of the protein sequence according to NP_005836.2.
  
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Predicted Homology Based on Immunogen Sequence: Human, Mouse, Rat
• Peptide Sequence: CNGQPSTLTIFETAL
• Concentration: 0.5 mg/ml
• Specificity: This antibody is expected to recognize isoform 1 (NP_005836.2) only.
• Application Info: P-ELISA 1:32000
  WB 1.0~3.0 ug/ml, 170 kDa band observed in rat renal cortex membranes.
  IHC 2.0~4.0 ug/ml. Shows strong cytoplasmic staining in small flat basal cells and weak cytoplasmic staining in the columnar secretory cells of the epithelium.
  IF Used successfully in human primary hepatocytes as describied in Xu et al, Am J Physiol Gastrointest Liver Physiol. 2010 Jul;200(1):G126-35, PMID: 20395535.
• Reference: * Schuetz JD, Connelly MC, Sun D, Paibir SG, Flynn PM, Srinivas RV, Kumar A, Fridland A.; MRP4: A previously unidentified factor in resistance to nucleoside-based antiviral drugs.; Nat Med. 1999 Sep;5(9):1048-51.; Pubmed 10470083
• Publications:

  Gori, I. et al. Augmented epithelial multidrug resistance-associated protein 4 expression in peritoneal endometriosis: regulation by lipoxin A(4). Fertil. Steril. 99, 1965-73.e2 (2013). 23472950

  Montani, M. et al. Multidrug resistance protein 4 (MRP4) expression in prostate cancer is associated with androgen signaling and decreases with tumor progression. Virchows Arch. 462, 437-43 (2013). 23503867

  Weerachayaphorn, J. et al. Nuclear factor-E2-related factor 2 is a major determinant of bile acid homeostasis in the liver and intestine. Am. J. Physiol. Gastrointest. Liver Physiol. 302, G925-36 (2012). 22345550

  Xu, S., Weerachayaphorn, J., Cai, S.-Y., Soroka, C. J. & Boyer, J. L. Aryl hydrocarbon receptor and NF-E2-related factor 2 are key regulators of human MRP4 expression. Am. J. Physiol. Gastrointest. Liver Physiol. 299, G126-35 (2010). 20395535

Target Info

• Gene Symbol: ABCC4
• Alias Symbols: MRP4, MOATB, MOAT-B
• NCBI Gene Id: 10257
• Protein Name: Multidrug resistance-associated protein 4
• Uniprot ID: O15439
• Protein Accession #: NP_005836.2
• Molecular Weight: 150 kDa