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Goat Anti-ABCC4 / MRP4 Antibody (OAEB02455)

Catalog#: OAEB02455
Domestic: within 1 week delivery International: 1 week
This product is Genway GWB-49EE5F
More Information
Predicted Species Reactivity Human, Rat
Product Format Liquid. Tris saline, pH7.3 with 0.02% sodium azide and 0.5% bovine serum albumin.
Clonality Polyclonal
Host Goat
Application IHC-P, P-WB, IF
Reconstitution and Storage Aliquot and store at -20C. Minimize freezing and thawing.
Replacement Item This antibody may replace item sc-20768 from Santa Cruz Biotechnology.
Immunogen Peptide with sequence CNGQPSTLTIFETAL, from the C Terminus of the protein sequence according to NP_005836.2.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Predicted Homology Based on Immunogen Sequence Human, Mouse, Rat
Complete computational species homology data ABCC4 Antibody (OAEB02455)
Concentration 0.5 mg/ml
Datasheets/Manuals Printable datasheet for anti-ABCC4 antibody - OAEB02455
Specificity This antibody is expected to recognize isoform 1 (NP_005836.2) only.
Application Info P-ELISA 1:32000
WB 1.0~3.0 ug/ml, 170 kDa band observed in rat renal cortex membranes.
IHC 2.0~4.0 ug/ml. Shows strong cytoplasmic staining in small flat basal cells and weak cytoplasmic staining in the columnar secretory cells of the epithelium.
IF Used successfully in human primary hepatocytes as describied in Xu et al, Am J Physiol Gastrointest Liver Physiol. 2010 Jul;200(1):G126-35, PMID: 20395535.
Target Reference * Schuetz JD, Connelly MC, Sun D, Paibir SG, Flynn PM, Srinivas RV, Kumar A, Fridland A.; MRP4: A previously unidentified factor in resistance to nucleoside-based antiviral drugs.; Nat Med. 1999 Sep;5(9):1048-51.; Pubmed 10470083

Gori, I. et al. Augmented epithelial multidrug resistance-associated protein 4 expression in peritoneal endometriosis: regulation by lipoxin A(4). Fertil. Steril. 99, 1965-73.e2 (2013). IHC-P, P-ELISA, WB, IF, Human, Rat 23472950

Montani, M. et al. Multidrug resistance protein 4 (MRP4) expression in prostate cancer is associated with androgen signaling and decreases with tumor progression. Virchows Arch. 462, 437-43 (2013). IHC-P, P-ELISA, WB, IF, Human, Rat 23503867

Weerachayaphorn, J. et al. Nuclear factor-E2-related factor 2 is a major determinant of bile acid homeostasis in the liver and intestine. Am. J. Physiol. Gastrointest. Liver Physiol. 302, G925-36 (2012). IHC-P, P-ELISA, WB, IF, Human, Rat 22345550

Xu, S., Weerachayaphorn, J., Cai, S.-Y., Soroka, C. J. & Boyer, J. L. Aryl hydrocarbon receptor and NF-E2-related factor 2 are key regulators of human MRP4 expression. Am. J. Physiol. Gastrointest. Liver Physiol. 299, G126-35 (2010). IHC-P, P-ELISA, WB, IF, Human, Rat 20395535

Gene Symbol ABCC4
Alias Symbols ABCC4, MRP4, MOATB, MOAT-B, EST170205, ATP-binding cassette, sub-family C (CFTR/MRP), member 4, canalicular multispecific organic anion transporter (ABC superfamily), Multidrug Resistance Protein 4, RP11-74A12.1, ATP-binding cassette, sub-family
NCBI Gene Id 10257
Protein Name Multidrug resistance-associated protein 4
Swissprot Id O15439
Protein Accession # NP_005836.2
Molecular Weight 150 kDa
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express ABCC4.
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express ABCC4.
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