Catalog No: OKEH06587
Price: $625.00
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityBos taurus, Bovine
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology Avp ELISA Kit (Bovine) (OKEH06587) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Avp has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Avp is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Avp captured in the well.
ELISA Kit Range78-5000pg/ml
ELISA Kit ReproducibilityIntra-assay reproducibility was evaluated with 20 replicates of 3 samples representing low, middle and high level LIPC. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level LIPC using 8 replicates on each of 3 plates.
Intra-Assay CV <10%
Inter-Assay CV <12%
ELISA Kit Component
Avp Microplate96 Wells (12 x 8 Well strips)
Avp Lyophilized Standard2 x 5 ng
100X Avp HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates
Sensitivity21 pg/mL
SpecificityNatural and recombinant Bovine Vasopressin-neurophysin 2-copeptin
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Gene SymbolAVP
Gene Full Namearginine vasopressin
Alias Symbolsarginine vasopressin (neurophysin II, antidiuretic hormone, diabetes insipidus, neurohypophyseal);arginine vasopressin-neurophysin II;AVP-NPII;neurophysin II;vasopressin-neurophysin 2-copeptin.
NCBI Gene Id280728
Protein NameP01180
Description of TargetVasopressin-neurophysin 2-copeptin
Uniprot IDhttps://www.uniprot.org/uniprot/P01180
Protein Accession #NP_789824
Nucleotide Accession #NM_176854
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