Catalog No: OKEH06587
Size:96 Wells
Price: $625.00
SKU
OKEH06587
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityBovine
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology Avp ELISA Kit (Bovine) (OKEH06587) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Avp has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Avp is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Avp captured in the well.
ELISA Kit Range78 - 5000pg/ml
ELISA Kit ReproducibilityIntra-assay reproducibility was evaluated with 20 replicates of 3 samples representing low, middle and high level LIPC. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level LIPC using 8 replicates on each of 3 plates.
Intra-Assay CV <10%
Inter-Assay CV <12%
ELISA Kit Component
ComponentAmount
Avp Microplate96 Wells (12 x 8 Well strips)
Avp Lyophilized Standard2 x 5 ng
100X Avp HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates
Sensitivity21 pg/mL
SpecificityNatural and recombinant Bovine Vasopressin-neurophysin 2-copeptin
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Gene SymbolAVP
Gene Full Nameguanine nucleotide binding protein (G protein), beta 3
Alias SymbolsAVP-NPII
NCBI Gene Id280728
Protein NameVasopressin-neurophysin 2-copeptin
Description of TargetNeurophysin 2 specifically binds vasopressin. Vasopressin has a direct antidiuretic action on the kidney, it also causes vasoconstriction of the peripheral vessels.
Swissprot IdP01180
Protein Accession #NP_789824
Nucleotide Accession #NM_176854
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express AVP.
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express AVP.
Write Your Own Review
You're reviewing:AVP ELISA Kit (Bovine) (OKEH06587)
Your Rating
We found other products you might like!