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Catalog No: OABB01687
Size:100UG
Price: $432.00
SKU
OABB01687
Availability: Domestic: within 1-2 week delivery | International: within 1-2 week delivery
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Datasheets/ManualsPrintable datasheet for GNB2L1 Antibody (OABB01687)
Product Info
Tested Species ReactivityHuman, Mouse, Rat
Predicted Species ReactivityBovine|Chicken|Dog|Horse|Human|Monkey|Mouse|Rabbit|Rat
Product FormatLyophilized. Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
ClonalityPolyclonal
ClonePolyclonal
IsotypeRabbit IgG
HostRabbit
ApplicationFlow cytometry|Immunocytochemistry|Immunofluorescence|Western blot
Additional InformationNotes: WB: The detection limit for RACK1 is approximately 0.25ng/lane under reducing conditions.
Tested Species: In-house tested species with positive results.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
::Background: Guanine nucleotide-binding protein subunit beta-2-like 1, also known as RACK1 is a 32kDa protein that in humans is encoded by the GNB2L1 gene. It is mapped to 5q35.3. GNB2L1 is a major component of translating ribosomes, which harbor significant amounts of PKC. This gene can provide a physical and functional link between PKC signaling and ribosome activation. GNB2L1 is also a mediator of agonist-induced Ca(2+) release. What’s more, GNB2L1 is an essential component of an oxygen-independent mechanism for regulating HIF1A stability. Overexpression of GNB2L1 and PKC-alpha suppressed CLOCK-BMAL1 transcriptional activity, and GNB2L1 stimulated phosphorylation of BMAL1 by PKC-alpha in vitro.
Reconstitution and Storage2°C to 8°C|-20°C
ImmunogenE.coli-derived human RACK1 recombinant protein (Position: T2-R317). Human RACK1 shares 100% amino acid (aa) sequence identity with both mouse and rat RACK1.
PurificationAffinity Purified
Concentration500 ug/ml
SpecificityNo cross reactivity with other proteins.
Application InfoWestern blot: 0.1-0.5 ug/ml: Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence: 2 ug/ml: Human
Flow Cytometry: 1-3 ug/1x10^6 cells: Human
Storage BufferEach vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
DescriptionRabbit IgG polyclonal antibody for Receptor of activated protein C kinase 1(RACK1) detection. Tested with WB, ICC/IF, FCM in Human;Mouse;Rat.
Gene SymbolRACK1
Gene Full Namereceptor for activated C kinase 1
Alias Symbolscell proliferation-inducing gene 21 protein;GNB2L1;Gnb2-rs1;guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1;guanine nucleotide binding protein beta polypeptide 2-like 1;guanine nucleotide-binding protein subunit beta-2-like 1;guanine nucleotide-binding protein subunit beta-like protein 12.3;H12.3;HLC-7;human lung cancer oncogene 7 protein;lung cancer oncogene 7;PIG21;proliferation-inducing gene 21;protein homologous to chicken B complex protein, guanine nucleotide binding;Receptor for activated C kinase;receptor of activated protein C kinase 1;receptor of activated protein kinase C 1;small ribosomal subunit protein RACK1.
NCBI Gene Id10399
Protein NameReceptor of activated protein C kinase 1
Description of TargetScaffolding protein involved in the recruitment, assembly and/or regulation of a variety of signaling molecules. Interacts with a wide variety of proteins and plays a role in many cellular processes. Component of the 40S ribosomal subunit involved in translational repression (PubMed:23636399). Involved in the initiation of the ribosome quality control (RQC), a pathway that takes place when a ribosome has stalled during translation, by promoting ubiquitination of a subset of 40S ribosomal subunits (PubMed:28132843). Binds to and stabilizes activated protein kinase C (PKC), increasing PKC-mediated phosphorylation. May recruit activated PKC to the ribosome, leading to phosphorylation of EIF6. Inhibits the activity of SRC kinases including SRC, LCK and YES1. Inhibits cell growth by prolonging the G0/G1 phase of the cell cycle. Enhances phosphorylation of BMAL1 by PRKCA and inhibits transcriptional activity of the BMAL1-CLOCK heterodimer. Facilitates ligand-independent nuclear translocation of AR following PKC activation, represses AR transactivation activity and is required for phosphorylation of AR by SRC. Modulates IGF1R-dependent integrin signaling and promotes cell spreading and contact with the extracellular matrix. Involved in PKC-dependent translocation of ADAM12 to the cell membrane. Promotes the ubiquitination and proteasome-mediated degradation of proteins such as CLEC1B and HIF1A. Required for VANGL2 membrane localization, inhibits Wnt signaling, and regulates cellular polarization and oriented cell division during gastrulation. Required for PTK2/FAK1 phosphorylation and dephosphorylation. Regulates internalization of the muscarinic receptor CHRM2. Promotes apoptosis by increasing oligomerization of BAX and disrupting the interaction of BAX with the anti-apoptotic factor BCL2L. Inhibits TRPM6 channel activity. Regulates cell surface expression of some GPCRs such as TBXA2R. Plays a role in regulation of FLT1-mediated cell migration. Involved in the transport of ABCB4 from the Golgi to the apical bile canalicular membrane (PubMed:19674157). Promotes migration of breast carcinoma cells by binding to and activating RHOA (PubMed:20499158).
Uniprot IDP63244
Molecular Weight35077 MW
  1. What is the species homology for "GNB2L1 Antibody (OABB01687)"?

    The tested species reactivity for this item is "Human, Mouse, Rat". This antibody is predicted to have homology to "Bovine|Chicken|Dog|Horse|Human|Monkey|Mouse|Rabbit|Rat".

  2. How long will it take to receive "GNB2L1 Antibody (OABB01687)"?

    This item is available "Domestic: within 1-2 week delivery | International: within 1-2 week delivery".

  3. What buffer format is "GNB2L1 Antibody (OABB01687)" provided in?

    This item is provided in "Lyophilized. Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.".
    Additional format options may be available. For more information please contact info@avivasysbio.com.

  4. What are other names for "GNB2L1 Antibody (OABB01687)"?

    This target may also be called "cell proliferation-inducing gene 21 protein;GNB2L1;Gnb2-rs1;guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1;guanine nucleotide binding protein beta polypeptide 2-like 1;guanine nucleotide-binding protein subunit beta-2-like 1;guanine nucleotide-binding protein subunit beta-like protein 12.3;H12.3;HLC-7;human lung cancer oncogene 7 protein;lung cancer oncogene 7;PIG21;proliferation-inducing gene 21;protein homologous to chicken B complex protein, guanine nucleotide binding;Receptor for activated C kinase;receptor of activated protein C kinase 1;receptor of activated protein kinase C 1;small ribosomal subunit protein RACK1." in publications.

  5. What is the shipping cost for "GNB2L1 Antibody (OABB01687)"?

    The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.

  6. What is the guarantee for "GNB2L1 Antibody (OABB01687)"?

    All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.

  7. Can I get bulk pricing for "GNB2L1 Antibody (OABB01687)"?

    You can get bulk pricing for this item by going here.

  8. What is the molecular weight of the protein?

    The molecular weight reported by Uniprot for this item is "35077 MW".
    Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.

  9. What protocols are available for "GNB2L1 Antibody (OABB01687)"?

    We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.

  10. What are positive controls for "RACK1"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  11. What are negative controls for "RACK1"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  12. What other proteins interact with "RACK1"?

    This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.

  13. What biological processes are associated with "RACK1"?

    This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.

  14. What cellular components are associated with "RACK1"?

    This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.

  15. What protein functions are associated with "RACK1"?

    This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.

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