GNB1 ELISA Kit (Rat) (OKEI00768)

Catalog No: OKEI00768

Size:96 Wells

Rat GNB1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology Gnb1 ELISA Kit (Rat) (OKEI00768) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Gnb1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Gnb1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of a luminol substrate which is catalyzed by the HRP to produce light emission. The light emission is read by a luminometer (or photo-multiplier equipped instrument) and the intensity of the emitted light is proportional to the amount of sample Gnb1
• ELISA Kit Range: 0.016 - 1 mIU/mL
• ELISA Kit Reproducibility: Intra-assay reproducibility was evaluated using 20 replicates of 3 samples representing low, middle and high level Gnb1. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level Gnb1 using 8 replicates on each of 3 plates.
  Intra-Assay CV <10%
  Inter-Assay CV <12%
• ELISA Kit Component:
  • LACTB2 Microplate: 96 Wells (12 x 8 Well strips)
  • LACTB2 Lyophilized Standard: 2
  • 100X LACTB2 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 10 mL
  • Conjugate Diluent: 1 x 10 mL
  • 25X Wash Buffer: 1 x 30 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Biological Fluids
• Sensitivity: 9.375μIU/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: GNB1
• Alias Symbols: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1, Transducin beta chain 1
• NCBI Gene Id: 24400
• Protein Name: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
• Description of Target: Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.
• Uniprot ID: P54311
• Protein Accession #: NP_112249
• Nucleotide Accession #: NM_030987