GNAT3 Antibody (OAEB00418)

Catalog#: OAEB00418

• Size: 100 ug

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human, Mouse, Rat, Dog
• Product Format: Liquid. Tris saline pH 7.3 with 0.5% BSA and 0.02% sodium azide.
• Clonality: Polyclonal
• Host: Goat
• Application: P-ELISA, IHC, WB
• Reconstitution and Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Gene Symbol: GNAT3
• NCBI Gene Id: 346562
• Protein Name: Guanine nucleotide-binding protein G(t) subunit alpha-3
• Swissprot Id: A8MTJ3
• Protein Accession #: NP_001095856.1
• Alias Symbols: GNAT3, guanine nucleotide binding protein, alpha transducing 3, tcag7.1053, GDCA, gustducin alpha-3
• Replacement Item: This antibody may replace item sc-12798 from Santa Cruz Biotechnology.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• RNA Seq: Find tissues and cell lines supported by RNA-seq analysis to express GNAT3.
• Immunogen: Peptide with sequence C-KNQFLDLNLKKEDKE, from the internal region of the protein sequence according to NP_001095856.1.
• Concentration: 0.5 mg/ml
• Application Info: P-ELISA: Antibody detection limit dilution 1:32000
  WB: Preliminary experiments gave an approx 27kDa band in Human Colon and Testis lysates and in cell lines NIH 3T3, A431 and Kelly after 0.1ug/ml antibody staining. Please note that no explanation can be currently found in literature, for the band observed was given the calculated size of 40.4kDa according to NP_001095856.1. The 27kDa band was successfully blocked by incubation with the immunizing peptide.
  IHC: In paraffin embedded Human Small Intestine shows staining of endocrine cells amongst the enterocytes. Recommended concentration, 3-6 ug/ml.

Protocol:

Tips Information:

Computational species homology for GNAT3 antibody (OAEB00418)