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GNAT3 Antibody (OAEB00418)

100 ug
$349.00
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Tested Species Reactivity:
Human
Predicted Species Reactivity:
Human, Mouse, Rat, Dog
Product Format:
Liquid. Tris saline pH 7.3 with 0.5% BSA and 0.02% sodium azide.
Clonality:
Polyclonal
Host:
Goat
Application:
P-ELISA, IHC, WB
Reconstitution and Storage:
Aliquot and store at -20°C. Minimize freezing and thawing.
Gene Symbol:
GNAT3
NCBI Gene Id:
346562
Protein Name:
Guanine nucleotide-binding protein G(t) subunit alpha-3
Swissprot Id:
A8MTJ3
Protein Accession #:
NP_001095856.1
Alias Symbols:
GNAT3, guanine nucleotide binding protein, alpha transducing 3, tcag7.1053, GDCA, gustducin alpha-3
Replacement Item:
This antibody may replace item sc-12798 from Santa Cruz Biotechnology.
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express GNAT3.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express GNAT3.
Immunogen:
Peptide with sequence C-KNQFLDLNLKKEDKE, from the internal region of the protein sequence according to NP_001095856.1.
Complete computational species homology data:
GNAT3 Antibody (OAEB00418)
Concentration:
0.5 mg/ml
Datasheets/Manuals:
Printable datasheet for anti-GNAT3 antibody - OAEB00418
Application Info:
P-ELISA: Antibody detection limit dilution 1:32000
WB: Preliminary experiments gave an approx 27kDa band in Human Colon and Testis lysates and in cell lines NIH 3T3, A431 and Kelly after 0.1ug/ml antibody staining. Please note that no explanation can be currently found in literature, for the band observed was given the calculated size of 40.4kDa according to NP_001095856.1. The 27kDa band was successfully blocked by incubation with the immunizing peptide.
IHC: In paraffin embedded Human Small Intestine shows staining of endocrine cells amongst the enterocytes. Recommended concentration, 3-6 ug/ml.

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77/03/2019 17:30
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Great antibody for IFA

This antibody works well in our recent human epithelial cell staining!!! We used this with 1:500 dilution in TNB buffer, and stained with AF594-anti-goat Ab as secondary!

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