- Tested Species Reactivity:
- Human
- Predicted Species Reactivity:
- Human, Mouse, Rat, Dog
- Product Format:
- Liquid. Tris saline pH 7.3 with 0.5% BSA and 0.02% sodium azide.
- Clonality:
- Polyclonal
- Host:
- Goat
- Application:
- P-ELISA, IHC, WB
- Reconstitution and Storage:
- Aliquot and store at -20°C. Minimize freezing and thawing.
- Gene Symbol:
- GNAT3
- NCBI Gene Id:
- 346562
- Protein Name:
- Guanine nucleotide-binding protein G(t) subunit alpha-3
- Swissprot Id:
- A8MTJ3
- Protein Accession #:
- NP_001095856.1
- Alias Symbols:
- GNAT3, guanine nucleotide binding protein, alpha transducing 3, tcag7.1053, GDCA, gustducin alpha-3
- Replacement Item:
- This antibody may replace item sc-12798 from Santa Cruz Biotechnology.
- Purification:
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Tissue Tool:
- Find tissues and cell lines supported by DNA array analysis to express GNAT3.
- RNA Seq:
- Find tissues and cell lines supported by RNA-seq analysis to express GNAT3.
- Immunogen:
- Peptide with sequence C-KNQFLDLNLKKEDKE, from the internal region of the protein sequence according to NP_001095856.1.
- Complete computational species homology data:
- GNAT3 Antibody (OAEB00418)
- Concentration:
- 0.5 mg/ml
- Datasheets/Manuals:
- Printable datasheet for anti-GNAT3 antibody - OAEB00418
- Application Info:
- P-ELISA: Antibody detection limit dilution 1:32000
WB: Preliminary experiments gave an approx 27kDa band in Human Colon and Testis lysates and in cell lines NIH 3T3, A431 and Kelly after 0.1ug/ml antibody staining. Please note that no explanation can be currently found in literature, for the band observed was given the calculated size of 40.4kDa according to NP_001095856.1. The 27kDa band was successfully blocked by incubation with the immunizing peptide.
IHC: In paraffin embedded Human Small Intestine shows staining of endocrine cells amongst the enterocytes. Recommended concentration, 3-6 ug/ml.
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