Catalog No: OKEH08012
Size:96T
Price: $725.00
SKU
OKEH08012
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.
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- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
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Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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Predicted Species Reactivity | Homo sapiens, Human | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology GNAS ELISA Kit (Human) (OKEH08012) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for GNAS has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for GNAS is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample GNAS captured in well. | ||||||||||||||||||||||
ELISA Kit Range | 0.312-20ng/mL | ||||||||||||||||||||||
ELISA Kit Recovery | Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 90% (n = 20) | ||||||||||||||||||||||
ELISA Kit Reproducibility | Mean Intra-assay CV%: <=7.6% (n = 20) Mean Inter-assay CV%: <=9.5% (n = 20) | ||||||||||||||||||||||
ELISA Kit Component |
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Additional Information | Function: Guanine nucleotide-binding proteins (G proteins) function as transducers in numerous signaling pathways controlled by G protein-coupled receptors (GPCRs). Signaling involves the activation of adenylyl cyclases, resulting in increased levels of the signaling molecule cAMP. GNAS functions downstream of several GPCRs, including beta-adrenergic receptors. XLas isoforms interact with the same set of receptors as GNAS isoforms. | ||||||||||||||||||||||
:: | Subcellular Location: Cell membrane Peripheral membrane protein Apical cell membrane | ||||||||||||||||||||||
:: | Unigene: Hs.125898 SMR: Q5JWF2 String: 9606.ENSP00000360141 | ||||||||||||||||||||||
:: | MIM: 139320 Pfam: PF00503 | ||||||||||||||||||||||
Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sensitivity | 0.128ng/mL | ||||||||||||||||||||||
Specificity | Natural and recombinant Human Guanine nucleotide-binding protein G(s) subunit alpha isoforms XLas | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
Gene Symbol | GNAS |
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Gene Full Name | GNAS complex locus |
Alias Symbols | adenylate cyclase-stimulating G alpha protein;AHO;alternative gene product encoded by XL-exon;C20orf45;extra large alphas protein;G protein subunit alpha S;GNAS1;GPSA;GSA;GSP;guanine nucleotide binding protein (G protein), alpha stimulating activity polypeptide 1;guanine nucleotide regulatory protein;NESP;PITA3;POH;protein ALEX;protein GNAS;protein SCG6 (secretogranin VI);SCG6;secretogranin VI;SgVI. |
NCBI Gene Id | 2778 |
Protein Name | Q5JWF2 |
Description of Target | Guanine nucleotide-binding protein G(s) subunit alpha isoforms XLas |
Uniprot ID | https://www.uniprot.org/uniprot/Q5JWF2 |
Protein Accession # | NP_001296812.1 |
Nucleotide Accession # | NM_001309883.1 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
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