GNAS Antibody (OAGA00440)

Catalog No: OAGA00440

Size:100 ul

GNAS antibody [C2C3], C-term detects GNAS protein by Western blot analysis.
A. 30 ug IMR32 whole cell lysate/extract
10 % SDS-PAGE
GNAS antibody [C2C3], C-term (OAGA00440) dilution: 1:500

Sample (50 ug of whole cell lysate)
A: Mouse brain
10% SDS PAGE
OAGA00440 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded OVCA, using GNAS(OAGA00440) antibody at 1:100 dilution.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid. 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: IHC-FFPE, WB
• Additional Information: Cellular Localization: Cell membrane; Peripheral membrane protein, Cell projection, ruffle, Cell membrane, Cell projection, ruffle
  Positive Controls: IMR32, mouse brain
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human GNAS. The exact sequence is proprietary.
  
• Purification: Purified by antigen-affinity chromatography.
• Predicted Homology Based on Immunogen Sequence: Rat, Xenopus laevis, Xenopus Tropicalis, Dog, Pig, Bovine
  
• Concentration: 0.78 mg/ml
• Application Info: WB: 1:500-1:3000
  IHC-FFPE: 1:100-1:1000
  
  Optimal working dilutions should be determined experimentally by the end user.

Target Info

• Gene Symbol: GNAS
• Gene Full Name: Neuroendocrine secretory protein 55
• Alias Symbols: AHO, GSA, GSP, POH, GPSA, NESP, SCG6, SgVI, GNAS1, PITA3, C20orf45
• NCBI Gene Id: 2778
• Protein Name: Neuroendocrine secretory protein 55
• Description of Target: This locus has a highly complex imprinted expression pattern. It gives rise to maternally, paternally, and biallelically expressed transcripts that are derived from four alternative promoters and 5' exons. Some transcripts contains a differentially methylated region (DMR) at their 5' exons, and this DMR is commonly found in imprinted genes and correlates with transcript expression. An antisense transcript is produced from an overlapping locus on the opposite strand. One of the transcripts produced from this locus, and the antisense transcript, are paternally expressed noncoding RNAs, and may regulate imprinting in this region. In addition, one of the transcripts contains a second overlapping ORF, which encodes a structurally unrelated protein - Alex. Alternative splicing of downstream exons is also observed, which results in different forms of the stimulatory G-protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Multiple transcript variants encoding different isoforms have been found for this gene. Mutations in this gene result in pseudohypoparathyroidism type 1a, pseudohypoparathyroidism type 1b, Albright hereditary osteodystrophy, pseudopseudohypoparathyroidism, McCune-Albright syndrome, progressive osseus heteroplasia, polyostotic fibrous dysplasia of bone, and some pituitary tumors.
• Uniprot ID: O95467
• Protein Accession #: NP_057676.1
• Molecular Weight: 28 kDa, 46kDa, 68kDa, 111kDa