GNAS Antibody - C-terminal region (OAAB09358)

Catalog No: OAAB09358

Size:400ul

Western blot analysis of GNAS (arrow) using rabbit polyclonal GNAS Antibody (C-term) (Cat. #OAAB09358). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GNAS gene.

GNAS Antibody (C-term) (Cat. #OAAB09358) western blot analysis in 293,NCI-H292 cell line lysates (35ug/lane).This demonstrates the GNAS antibody detected the GNAS protein (arrow).

GNAS Antibody (C-term) (Cat. #OAAB09358)immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GNAS Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

GNAS Antibody (C-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Confocal immunofluorescent analysis of GNAS Antibody (C-term)(Cat#OAAB09358) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Host: Rabbit
• Application: WB, IHC-P, IF, FC
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This GNAS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 287-315 amino acids from the C-terminal region of human GNAS.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Predicted Homology Based on Immunogen Sequence: Mouse, Rat, Bovine, Hamster, Pig
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: WB: 1:1000
  IHC-P: 1:10~50
  IF: 1:10~50
  FC: 1:10~50

Target Info

• Alias Symbols: GNAS
• Protein Name: GNAS complex locus EMBL AAH89157.2
• Description of Target: Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The Gs protein is involved in hormonal regulation of adenylate cyclase: it activates the cyclase in response to beta-adrenergic stimuli. Alternative splicing of downstream exons of the GNAS gene is observed, which results in different forms of the stimulatory G protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Multiple transcript variants have been found for this gene, but the full-length nature and/or biological validity of some variants have not been determined. Mutations in this gene result in pseudohypoparathyroidism type 1a, pseudohypoparathyroidism type 1b, Albright hereditary osteodystrophy, pseudopseudohypoparathyroidism, McCune-Albright syndrome, progressive osseus heteroplasia, polyostotic fibrous dysplasia of bone, and some pituitary tumors.
• Uniprot ID: Q5FWY2
• Molecular Weight: 44 kDa