GNAO1 Antibody (OAGA04665)

Catalog No: OAGA04665

Size:100 ul

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GNAO1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GNAO1 antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GNAO1 antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

GNAO1 antibody detects GNAO1 protein at cytoplasm on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
GNAO1 antibody dilution: 1:500.

Immunofluorescence analysis of methanol-fixed HeLa, using GNAO1 antibody at 1:200 dilution.

Immunoprecipitation of GNAO1 protein from HeLa whole cell extracts using 5 ug of GNAO1 antibody .
Western blot analysis was performed using GNAO1 antibody .
EasyBlot anti-Rabbit IgG was used as a secondary reagent.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-P, IP, WB
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human GNAO1. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1 mg/ml
• Preservative: 0.01% Thimerosal
• Application Info: Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, IHC-P: 1:100-1:1000, IP: 1:100-1:500, WB: 1:500-1:20000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: GNAO1
• Gene Full Name: G protein subunit alpha o1
• Alias Symbols: GNAO, DEE17, NEDIM, EIEE17, HLA-DQB1, G-ALPHA-o
• NCBI Gene Id: 2775
• Protein Name: guanine nucleotide-binding protein G(o) subunit alpha
• Description of Target: The protein encoded by this gene represents the alpha subunit of the Go heterotrimeric G-protein signal-transducing complex. Defects in this gene are a cause of early-onset epileptic encephalopathy. Two transcript variants encoding different isoforms have been found for this gene.
• Uniprot ID: P09471
• Protein Accession #: NP_066268.1
• Nucleotide Accession #: NM_020988.2