Catalog No: OKCD00454 (Formerly GWB-KBBQH8)
Size:96WELLS
Price: $675.00
SKU
OKCD00454
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Datasheets/ManualsPrintable datasheet for GNA11 ELISA Kit (Human) (OKCD00454)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
92-101%94-105%86-94%91-104%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to G Protein Alpha 11 (GNa11). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to G Protein Alpha 11 (GNa11). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain G Protein Alpha 11 (GNa11), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of G Protein Alpha 11 (GNa11) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
82-9992
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level G Protein Alpha 11 (GNa11) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level G Protein Alpha 11 (GNa11) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-GNA11 Microplate96 Wells (12 x 8 Well Strips)
GNA11 Lyophilized Standard2 x 10 ng
100X Biotinylated GNA11 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids.
Sensitivity< 0.059 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of G Protein Alpha 11 (GNa11). No significant cross-reactivity or interference between G Protein Alpha 11 (GNa11) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolGNA11
Gene Full NameG protein subunit alpha 11
Alias SymbolsFBH;FBH2;FHH2;g alpha-11;GNA-11;guanine nucleotide binding protein (G protein), alpha 11 (Gq class);guanine nucleotide-binding protein G(y) subunit alpha;guanine nucleotide-binding protein subunit alpha-11;HHC2;HYPOC2.
NCBI Gene Id2767
Protein NameGuanine nucleotide-binding protein subunit alpha-11
Description of TargetGuanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Acts as an activator of phospholipase C.
Uniprot IDP29992
Protein Accession #NP_002058
Nucleotide Accession #NM_002067.4
Protein Size (# AA)359
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