GML ELISA Kit (Human) (OKCD00518)

Catalog No: OKCD00518 (Formerly GWB-KBBZF2)

Size:96WELLS

Human GML ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glycosylphosphatidylinositol Anchored Molecule Like Protein (GML). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glycosylphosphatidylinositol Anchored Molecule Like Protein (GML). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glycosylphosphatidylinositol Anchored Molecule Like Protein (GML), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Glycosylphosphatidylinositol Anchored Molecule Like Protein (GML) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glycosylphosphatidylinositol Anchored Molecule Like Protein (GML) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glycosylphosphatidylinositol Anchored Molecule Like Protein (GML) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-GML Microplate: 96 Wells (12 x 8 Well Strips)
  • GML Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated GML Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates and other biological fluids.
• Sensitivity: < 0.055 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Glycosylphosphatidylinositol Anchored Molecule Like Protein (GML). No significant cross-reactivity or interference between Glycosylphosphatidylinositol Anchored Molecule Like Protein (GML) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: GML
• Gene Full Name: glycosylphosphatidylinositol anchored molecule like
• Alias Symbols: glycosylphosphatidylinositol anchored molecule like protein;glycosyl-phosphatidylinositol-anchored molecule-like protein;Glycosylphosphatidylinositol-anchored molecule-like protein;GPI anchored molecule like protein;LY6DL.
• NCBI Gene Id: 2765
• Protein Name: Glycosyl-phosphatidylinositol-anchored molecule-like protein
• Description of Target: May play a role in the apoptotic pathway or cell-cycle regulation induced by p53/TP53 after DNA damage.
• Uniprot ID: Q99445
• Protein Accession #: NP_002057
• Nucleotide Accession #: NM_002066.2
• Protein Size (# AA): 158