GMCSF ELISA Kit (Dog) (OKCD04412)

Catalog No: OKCD04412

Size:96WELLS

Dog GMCSF ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Canis lupus|Dog
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	84-98%	87-103%	80-91%	95-105%
  EDTA Plasma (n=5)	80-104%	85-93%	96-104%	79-88%
  Heparin Plasma (n=5)	84-94%	83-103%	81-91%	90-102%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 7.8-500pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average (%)
  Serum (n=5)	81-105	86
  EDTA Plasma (n=5)	81-92	88
  Heparin Plasma (n=5)	96-103	101

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Gmcsf Microplate: 96 Wells (12 x 8 Well strips)
  • Gmcsf Lyophilized Standard: 2
  • 100X Gmcsf HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Sensitivity: 3.1 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CSF2
• Gene Full Name: colony stimulating factor 2
• Alias Symbols: colony-stimulating factor;CSF;GM-CSF;granulocyte-macrophage colony-stimulating factor.
• NCBI Gene Id: 403923
• Protein Name: Granulocyte-macrophage colony-stimulating factor
• Description of Target: Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
• Uniprot ID: P48749
• Protein Accession #: NP_001003245.1
• Nucleotide Accession #: NM_001003245.1