Catalog No: OKCD00603 (Formerly GWB-KBBMH8)
Size:96WELLS
Price: $750.00
SKU
OKCD00603
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Datasheets/ManualsPrintable datasheet for GM2A ELISA Kit (Human) (OKCD00603)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)90-97%85-96%94-104%93-105%
EDTA Plasma (n=5)80-91%81-96%93-104%78-95%
Heparin Plasma (n=5)92-101%78-105%85-103%79-99%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to GM2 Ganglioside Activator (GM2A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to GM2 Ganglioside Activator (GM2A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GM2 Ganglioside Activator (GM2A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of GM2 Ganglioside Activator (GM2A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)98-105102
EDTA plasma(n=5)78-10482
heparin plasma(n=5)84-9490
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level GM2 Ganglioside Activator (GM2A) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level GM2 Ganglioside Activator (GM2A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-GM2A Microplate96 Wells (12 x 8 Well Strips)
GM2A Lyophilized Standard2 x 10 ng
100X Biotinylated GM2A Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity< 0.064 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of GM2 Ganglioside Activator (GM2A). No significant cross-reactivity or interference between GM2 Ganglioside Activator (GM2A) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolGM2A
Gene Full NameGM2 ganglioside activator
Alias Symbolscerebroside sulfate activator protein;ganglioside GM2 activator;GM2-AP;SAP-3;shingolipid activator protein 3;sphingolipid activator protein 3.
NCBI Gene Id2760
Protein NameGanglioside GM2 activator
Description of TargetThe large binding pocket can accommodate several single chain phospholipids and fatty acids, GM2A also exhibits some calcium-independent phospholipase activity (By similarity). Binds gangliosides and stimulates ganglioside GM2 degradation. It stimulates only the breakdown of ganglioside GM2 and glycolipid GA2 by beta-hexosaminidase A. It extracts single GM2 molecules from membranes and presents them in soluble form to beta-hexosaminidase A for cleavage of N-acetyl-D-galactosamine and conversion to GM3.
Uniprot IDP17900
Protein Accession #NP_000396
Nucleotide Accession #NM_000405.4
Protein Size (# AA)193
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