GM CSF Protein (OPPA00487)

Catalog No: OPPA00487 (Formerly GWB-C02CF4)

Size:5UG

Product Info

• Predicted Species Reactivity: Mouse
• Product Format: GM-CSF Mouse was lyophilized with no additives. Physical appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Host: E. Coli
• Additional Information: Solubility: It is recommended to reconstitute the lyophilized Granulocyte Macrophage Colony Stimulating Factor in sterile 20mM AcOH (acetic Acid) not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
  Biological Activity: The ED50 as determined by the dose-dependant stimulation of the proliferation of murine FDC-P1 cell line is < 0.2 ng/ml, corresponding to a Specific Activity of 5x10⁶ IU/mg.
  Protein Content: GM-CSF quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.765 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of GM-CSF as a Reference Standard.
  Protein Content: GM-CSF quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.765 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of GM-CSF as a Reference Standard.
  Product Introduction: GMCSF is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13.GM-CSF stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
  Product Description: Granulocyte Macrophage Colony Stimulating Factor Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 125 amino acids and having a molecular mass of 14285.35 Dalton.GM-CSF Mouse is purified by proprietary chromatographic techniques.
• Reconstitution and Storage: Lyophilized Granulocyte Macrophage Colony Stimulating Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution GM-CSF should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0. 1% HSA or BSA). Please prevent freeze-thaw cycles.
• Purity: Greater than 98.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Peptide Sequence: The sequence of the first five N - terminal amino acids was determined and was found to be Met - Ala - Pro - Thr - Arg.

Target Info

• Gene Symbol: mGM CSF
• Alias Symbols: CSF, Csfg, GMCS, Csfgm, GMCSF, Gm-CS, MGI-I, Gm-CSf, MGI-IGM
• NCBI Gene Id: 12981
• Protein Name: Granulocyte-macrophage colony-stimulating factor
• Description of Target: Recombinant Mouse Granulocyte Macrophage-Colony Stimulating Factor
• Uniprot ID: P01587
• Protein Accession #: NP_034099.2
• Protein Size (# AA): Recombinant